A competitive immunosensor for ultrasensitive detection of sulphonamides from environmental waters using silver nanoparticles decorated single-walled carbon nanohorns as labels. (June 2019)

• Record Type: Journal Article
• Authors: Zhang, Zhen; Yang, Mingyue; Wu, Xiangyang; Dong, Shuaibing; Zhu, Nuanfei; Gyimah, Eric; Wang, Kun; Li, Yong
• Is Part Of: Chemosphere. Volume 225(2019)
• Page Start: 282
• Page End: 287
• Publication Date: 2019-06
• Subjects: Electrochemical immunosensor -- Sulphonamides -- Au nanodendrites -- Single-walled carbon nanohorns -- Silver nanoparticles; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2019.03.033
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: A novel competitive electrochemical immunosensor based on Au nanodendrites (Au NDs)/silver nanoparticles (Ag NPs) @single-walled carbon nanohorns (SWCNHs) was established for sensitive determination of sulphonamides (SAs) in aquatic environments. The indirectly competitive binding system of the approach was composed of coating antigen that coated on Au NDs/glass carbon electrode (GCE), the target and primary antibody (Ab1 ). When Ab2 @Ag NPs@SWCNHs was captured by coating antigen (Cag)- Ab1 complex, massive Ag + will be released from electrode in the presence nitric acid (HNO3 ), consequently, the generated Ag + will significantly amplify the electrochemical signal, which would be recorded by the linear sweep voltametry (LSV). Meanwhile, the used three-dimensional Au nanodendrites (Au NDs) could increase the conductivity of the electrode and the size of the active surface area to improve the antigen-loading. Under the optimal conditions, the immunosensor showed a good linear relationship for sulfamethazine (SMZ)ranged in 0.33–63.81 ng/mL with a detection limit of 0.12 ng/mL (LOD, based on 90% inhibition). In addition, the proposed approach exhibited satisfactory accuracy and precision (recoveries, 79.25–119.25%; CV, 2.14–9.58%), it can be applied for rapid analysis of the trace pollutants from environmental waters. Highlights: An electrochemical immunosensor was fabricated for sensitive determination of SAs. The signal amplification strategy was based on Ag NPs dissolution under HNO3 . The established immunosensor exhibited satisfactory accuracy and good sensitivity. The method showed great potential to monitor SAs in environmental waters.