Evaluation of preimplantation genetic testing based on next-generation sequencing for balanced reciprocal translocation carriers. Issue 5 (May 2019)

• Record Type: Journal Article
• Authors: Cai, Yunni; Ding, Min; Lin, Fei; Diao, Zhenyu; Zhang, Ningyuan; Sun, Haixiang; Zhou, Jianjun
• Is Part Of: Reproductive biomedicine online. Volume 38:Issue 5(2019)
• Page Start: 669
• Page End: 675
• Publication Date: 2019-05
• Subjects: Balanced reciprocal translocation -- Copy number variation sequencing -- Frozen embryo transfer -- Gonadotrophin-releasing hormone -- Next-generation sequencing -- Preimplantation genetic testing; Human reproductive technology -- Periodicals; Human embryo -- Periodicals; Reproduction -- Periodicals; 616.692
• DOI: 10.1016/j.rbmo.2018.12.043
• Languages: English
• ISSNs: 1472-6483

Abstract

Abstract: Research question: Can next-generation sequencing (NGS) based on copy number variation sequencing (CNV-Seq) identify normal/balanced embryos in balanced reciprocal translocation carriers and what are their reproductive outcomes? Design: One hundred couples with balanced reciprocal translocation who underwent a total of 134 preimplantation genetic testing (PGT) cycles between January 2015 and October 2017 were evaluated. Trophectoderm cells of blastocysts were biopsied for CNV-Seq-based NGS. All the balanced/normal blastocysts were vitrified and cryopreserved. Single balanced/normal blastocysts were warmed and transferred in the subsequent frozen embryo transfer (FET) cycle. Results: During the study period, 400 blastocysts were analysed by NGS-PGT, of which 109 (27.25%) were balanced and euploid. A total of 52 blastocysts were transferred in the FET cycle. Clinical pregnancy was confirmed in 34 women (65.38%), with a miscarriage rate of 2.94%; 26 healthy term babies were born, including 24 singletons and one set of twins, while eight couples had ongoing pregnancies. Amniocentesis revealed a fetal chromosome status that was consistent with the NGS-PGT results. Female carriers had a significantly higher blastocyst rate than did the male carriers (37.01% versus 31.27%, P = 0.04). The transferable blastocyst rate was higher in couples treated with gonadotrophin-releasing hormone (GnRH) antagonist than in those treated with GnRH agonist (38.20% versus 24.37%, P = 0.01). However, neither carrier sex nor ovarian stimulation protocol influenced the clinical pregnancy rate. Conclusions: CNV-Seq-based NGS is an efficient and reliable PGT method for balanced reciprocal translocation.