Genome-wide Identification and analysis of the stress-resistance function of the TPS (Trehalose-6-Phosphate Synthase) gene family in cotton. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Mu, Min; Lu, Xu-Ke; Wang, Jun-Juan; Wang, De-Long; Yin, Zu-Jun; Wang, Shuai; Fan, Wei-Li; Ye, Wu-Wei
• Is Part Of: BMC genetics. Volume 17:Issue 1(2016)
• Page Start: 1
• Page End: 11
• Publication Date: 2016-12
• Subjects: Rehalose-6-phosphase synthase (TPS) -- Gossypium raimondii -- Gossypium arboretum -- Gossypium hirsutum L -- Gene family; Genetics -- Periodicals; 576.505
• DOI: 10.1186/s12863-016-0360-y
• Languages: English
• ISSNs: 1471-2156

Abstract

Abstract Background Trehalose (a-D-glucopyranosyl a-D-glucopyranoside) is a nonreducing disaccharide and is widely distributed in bacteria, fungi, algae, plants and invertebrates. In the study, the identification of trehalose-6-phosphate synthase (TPS ) genes stress-related in cotton, and the genetic structure analysis and molecular evolution analysis ofTPSs were conducted with bioinformatics methods, which could lay a foundation for further research ofTPS functions in cotton. Results The genome information ofGossypium raimondii (group D), G. arboreum L. (group A), andG. hirsutum L. (group AD) was used in the study. Fifty-threeTPSs were identified comprising 15 genes in group D, 14 in group A, and 24 in group AD. Bioinformatics methods were used to analyze the genetic structure and molecular evolution ofTPSs . Real-time PCR analysis was performed to investigate the expression patterns of gene family members. AllTPS family members in cotton can be divided into two subfamilies: Class I and Class II. The similarity of theTPS sequence is high within the same species and close within their family relatives. The genetic structures of twoTPS subfamily members are different, with more introns and a more complicated gene structure in Class I. There is a TPS domain(Glyco transf_20) at the N-terminal in allTPS family members and a TPP domain(Trehalose_PPase) at the C-terminal in all exceptGrTPS6, GhTPS4, andGhTPS9 . All Class II members contain a UDP-forming domain. The responses to environmental stresses showed that stresses could induce the expression ofTPSs but the expression patterns vary with different stresses. Conclusions The distribution ofTPS s varies with different species but is relatively uniform on chromosomes. Genetic structure varies with different gene members, and expression levels vary with different stresses and exhibit tissue specificity. The upregulated genes in upland cotton TM-1 is significantly more than that inG. raimondii andG. arboreum L.Shixiya 1.