Molecular characterization and evaluation of the emerging antibiotic-resistant Streptococcus pyogenes from eastern India. Issue 1 (December 2016) - Ray, Dipanwita
Abstract Background Group A Streptococcus strains causing wide variety of diseases, recently became noticeable in eastern India, are not amenable to standard treatment protocol thus enhancing the possibility of disease morbidity by becoming antibiotic resistance. Methods The association of Lancefield group A Streptococcal variation with degree ofvir architectural diversity was evaluated usingemm typing and restriction fragment length polymorphism analyses. The antibiotic sensitivity patterns were examined by modified Kirby-Bauer method of disk diffusion. Percentage calculations, 95% confidence interval and one-way ANOVA were used to assess differences in proportions. Results Our observations revealed 20 differentemm types and 13 differentHaeIII vir typing patterns. A 1.2 kb fragment was found in allHaeIII typing pattern. Fragments of 1.2 kb and 550 bp were conserved in majority of the isolates.HinfI digestion was found proficient in differentiating the strains of samevir typing patterns. Strong predominance ofspeC (85%) andspeF (80%) genes have been observed encoding exotoxins production. 4 isolates were found to be erythromycin resistant and were of genotypeemm 49. High degree of tetracycline resistance was shown by 53.57% isolates which belonged to 12 differentemm genotypes. Conclusions These findings suggested that in addition toemm typing, sequential application ofHaeIII andHinfI restriction enzymes invir typing analysis is an effective tool for group A streptococcal molecular characterization associated with antibiotic resistance.