A novel human leiomyoma tissue derived matrix for cell culture studies. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Salo, Tuula; Sutinen, Meeri; Hoque Apu, Ehsanul; Sundquist, Elias; Cervigne, Nilva; de Oliveira, Carine; Akram, Saad; Ohlmeier, Steffen; Suomi, Fumi; Eklund, Lauri; Juusela, Pirjo; Åström, Pirjo; Bitu, Carolina; Santala, Markku; Savolainen, Kalle; Korvala, Johanna; Paes Leme, Adriana; Coletta, Ricardo
• Is Part Of: BMC cancer. Volume 15:Issue 1(2015)
• Page Start: 1
• Page End: 16
• Publication Date: 2015-12
• Subjects: Tumor microenvironment matrix -- Invasion -- Migration -- Hanging drop -- Colony formation -- Spheroid formation -- Capillary formation; Cancer -- Periodicals; 616.994005
• DOI: 10.1186/s12885-015-1944-z
• Languages: English
• ISSNs: 1471-2407

Abstract

Abstract Background The composition of the matrix molecules is important inin vitro cell culture experiments ofe.g . human cancer invasion and vessel formation. Currently, the mouse Engelbreth-Holm-Swarm (EHS) sarcoma -derived products, such as Matrigel®, are the most commonly used tumor microenvironment (TME) mimicking matrices for experimental studies. However, since Matrigel® is non-human in origin, its molecular composition does not accurately simulate human TME. We have previously described a solid 3D organotypic myoma disc invasion assay, which is derived from human uterus benign leiomyoma tumor. Here, we describe the preparation and analyses of a processed, gelatinous leiomyoma matrix, named Myogel. Methods A total protein extract, Myogel, was formulated from myoma. The protein contents of Myogel were characterized and its composition and properties compared with a commercial mouse Matrigel®. Myogel was tested and compared to Matrigel® in human cell adhesion, migration, invasion, colony formation, spheroid culture and vessel formation experiments, as well as in a 3D hanging drop video image analysis. Results We demonstrated that only 34 % of Myogel's molecular content was similar to Matrigel®. All test results showed that Myogel was comparable with Matrigel®, and when mixed with low-melting agarose (Myogel-LMA) it was superior to Matrigel® inin vitro Transwell® invasion and capillary formation assays. Conclusions In conclusion, we have developed a novel Myogel TME matrix, which is recommended forin vitro human cell culture experiments since it closely mimics the human tumor microenvironment of solid cancers.