Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Stern, Daniel; Olson, Victoria; Smith, Scott; Pietraszczyk, Marko; Miller, Lilija; Miethe, Peter; Dorner, Brigitte; Nitsche, Andreas
• Is Part Of: Virology journal. Volume 13:Issue 1(2016)
• Page Start: 1
• Page End: 7
• Publication Date: 2016-12
• Subjects: Orthopoxvirus -- Variola virus -- Monkeypox virus -- Cowpox virus -- Vaccinia virus -- bioterrorism -- Zoonosis -- Point-of-care -- Rapid detection -- ABICAP; Virology -- Periodicals; 579.2
• DOI: 10.1186/s12985-016-0665-5
• Languages: English
• ISSNs: 1743-422X

Abstract

Abstract Background The rapid and reliable detection of infectious agents is one of the most challenging tasks in scenarios lacking well-equipped laboratory infrastructure, like diagnostics in rural areas of developing countries. Commercially available point-of-care diagnostic tests for emerging and rare diseases are particularly scarce. Results In this work we present a point-of-care test for the detection ofOrthopoxviruses (OPV). The OPV ABICAP assay detects down to 1 × 104 plaque forming units/mL of OPV particles within 45 min. It can be applied to clinical material like skin crusts and detects all zoonotic OPV infecting humans, includingVaccinia, Cowpox, Monkeypox, and most importantlyVariola virus . Conclusions Given the high sensitivity and the ease of handling, the novel assay could be highly useful for on-site diagnostics of suspectedMonkeypox virus infections in areas lacking proper laboratory infrastructure as well as rapid on-site testing of suspected bioterrorism samples.