Effect of the saliva from different triatomine species on the biology and immunity of TLR-4 ligand and Trypanosoma cruzi-stimulated dendritic cells. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Mendes, Maria; Carvalho-Costa, Tamires; da Silva, Marcos; Anhê, Ana; Guimarães, Rafaela; da Costa, Thiago; Ramirez, Luis; Rodrigues, Virmondes; Oliveira, Carlo
• Is Part Of: Parasites & vectors. Volume 9:Issue 1(2016)
• Page Start: 1
• Page End: 14
• Publication Date: 2016-12
• Subjects: Triatomines -- Saliva -- Dendritic cells -- Trypanosoma cruzi -- Immunomodulation; Parasitism -- Periodicals; Parasites -- Periodicals; Vector-pathogen relationships -- Periodicals; Animals as carriers of disease -- Periodicals; Insects as carriers of disease -- Periodicals; 616.96
• DOI: 10.1186/s13071-016-1890-x
• Languages: English
• ISSNs: 1756-3305

Abstract

Abstract Background Triatomines are blood-sucking vectors ofTrypanosoma cruzi, the causative agent of Chagas disease. During feeding, triatomines surpass the skin host response through biomolecules present in their saliva. Dendritic cells (DCs) play a crucial role in the induction of the protection to aggressive agents, including blood-sucking arthropods. Here, we evaluated if salivary components of triatomines from different genera evade the host immunity by modulating the biology and the function of LPS- orT. cruzi -stimulated DCs. Methods Saliva ofPanstrongylus lignarius, Meccus pallidipennis, Triatoma lecticularia andRhodnius prolixus were obtained by dissection of salivary glands and the DCs were obtained from the differentiation of mouse bone marrow precursors. Results The differentiation of DCs was inhibited by saliva of all species tested. Saliva differentially inhibited the expression of MHC-II, CD40, CD80 and CD86 in LPS-matured DCs. Except for the saliva ofR. prolixus, which induced IL-6 cytokine production, TNF-α, IL-12 and IL-6 were inhibited by the saliva of the other three tested species and IL-10 was increased in all of them. Saliva per se, also induced the production of IL-12, IL-6 and IL-10. Only the saliva ofR. prolixus induced DCs apoptosis. The presence of PGE2 was not detected in the saliva of the four triatomines studied. Finally, T. cruzi invasion on DCs is enhanced by the presence of the triatomine saliva. Conclusions These results demonstrate that saliva from different triatomine species exhibit immunomodulatory effects on LPS andT. cruzi -stimulated DCs. These effects could be related to hematophagy and transmission ofT. cruzi during feeding.