MiR-491 regulates glioma cells proliferation by targeting TRIM28 in vitro. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Qi, Zengxin; Cai, Shengyong; Cai, Jiajun; Chen, Lingchao; Yao, Yu; Chen, Liang; Mao, Ying
• Is Part Of: BMC neurology. Volume 16:Issue 1(2016)
• Page Start: 1
• Page End: 7
• Publication Date: 2016-12
• Subjects: Glioblastoma -- miR-491 -- TRIM28 -- Proliferation; Neurology -- Periodicals; 616.8005
• DOI: 10.1186/s12883-016-0769-y
• Languages: English
• ISSNs: 1471-2377

Abstract

Abstract Background MicroRNAs are significantly involved in tumorigenesis and progression of glioma. However, the critical part they play in glioma have not been fully elaborated. miR-491 and Tripartite motif containing 28 (TRIM28) are reported to aberrantly express in glioblastoma multiforme (GBM). Here, we detected miR-491 and TRIM28 expression and function in glioma cells. Methods We analyzed miR-491 expressions in 20 primary human GBM tissues and 6 control brain tissues by qRT-PCR assays and searched for The Cancer Genome Atlas (TCGA) database. Then we predicted possible mRNA target of miR-491 by TargetScan/MicroRNA and confirmed it via luciferase reporter assays. Knock-down of miR-491 and transfection of pLenti-TRIM28 were performed in U251 and U87 cells. Proliferation ability was examined by MTT and clone formation assays. Results miR-491 expression was obviously reduced in GBM cells and tissues. There was a positive correlation between the down-regulation of miR-491 and poor prognosis. Spearman's correlation analysis demonstrated that miR-491 expression was negatively correlated with TRIM28 protein level. Possible mRNA binding sites of miR-491 predicted by TargetScan/MicroRNA were proved by luciferase assays. Clone formation and MTT assays indicated that up-regulation of miR-491 inhibited the proliferation of glioma cells. Conclusions miR-491 regulates glioma cells proliferation in vitro by targeting TRIM28.