Development and progress for three decades in umu test systems. (December 2016)
- Record Type:
- Journal Article
- Title:
- Development and progress for three decades in umu test systems. (December 2016)
- Main Title:
- Development and progress for three decades in umu test systems
- Authors:
- Oda, Yoshimitsu
- Abstract:
- Abstract Umu test have been widely used to predict the detection and assessment of DNA- damaging chemicals in environmental genotoxicity field for three decades. This test system is more useful with respect to simplicity, sensitivity, rapidity, and reproducibility. A review of the literature on the development of theumu test is presented in this article. The contents of this article are included a description of numerous data using theumu test. This test have been fully evaluated and used in many directions. Different genetically engineeredumu systems introducing bacterial and rat or human drug metabolizing enzymes into theumu tester strains, have been successfully established and are considered as useful tools for genotoxicity assays to study the mechanisms of biotransformation in chemical carcinogenesis. Actually, we developed that two types of bacterial metabolizing enzymes and 4 types of rat and human metabolizing enzyme DNAs are expressed in these strains such as nitroreductase andO -acetyltransferase, cytochrome P450, N -acetyltransferases, sulfotransferases, and glutathioneS -transferases, respectively. Due to increasing numbers of minute environmental samples and new pharmaceuticals, a high-throughputumu test system usingSalmonella typhimurium TA1535/pSK1002, NM2009, and NM3009 strains provides a useful for these genotoxicity screening. I also briefly describe the first attempts to incorporate suchumu tester strain into photo-genotoxicity test.
- Is Part Of:
- Genes and environment. Volume 38:Number 1(2016)
- Journal:
- Genes and environment
- Issue:
- Volume 38:Number 1(2016)
- Issue Display:
- Volume 38, Issue 1 (2016)
- Year:
- 2016
- Volume:
- 38
- Issue:
- 1
- Issue Sort Value:
- 2016-0038-0001-0000
- Page Start:
- 1
- Page End:
- 14
- Publication Date:
- 2016-12
- Subjects:
- umu test -- SOS response -- Metabolic activation -- Genotoxicity -- Cytochrome P450 -- Glutathione S-transferase -- O-acetyltransferase -- Sulfotransferase
Mutagenesis -- Periodicals
Pollution -- Environmental aspects -- Periodicals
Mutagens -- Periodicals
Mutation (Biology) -- Periodicals
Genetic toxicology -- Periodicals
Genetic toxicology
Mutagenesis
Mutagens
Mutation (Biology)
Pollution -- Environmental aspects
Periodicals
572.838 - Journal URLs:
- http://www.genesenvironment.com/ ↗
http://www.jstage.jst.go.jp/browse/jemsge/ ↗
http://www.jstage.jst.go.jp/browse/jemsge/_vols ↗
http://link.springer.com/ ↗ - DOI:
- 10.1186/s41021-016-0054-8 ↗
- Languages:
- English
- ISSNs:
- 1880-7062
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 9964.xml