Immunomodulation in the canine endometrium by uteropathogenic Escherichia coli. (December 2016)

• Record Type: Journal Article
• Authors: Henriques, Sofia; Silva, Elisabete; Silva, Marta; Carvalho, Sandra; Diniz, Patrícia; Lopes-da-Costa, Luís; Mateus, Luisa
• Is Part Of: Veterinary research. Volume 47:Number 1(2016)
• Page Start: 1
• Page End: 17
• Publication Date: 2016-12
• Subjects: Veterinary medicine -- Periodicals; Veterinary medicine -- France -- Periodicals; 636.089
• DOI: 10.1186/s13567-016-0396-z
• Languages: English
• ISSNs: 0928-4249

Abstract

Abstract This study was designed to evaluate the role ofE. coli α-hemolysin (HlyA) in the pathogenesis of canine pyometra, and on the immune response of canine endometrial epithelial and stromal cells. In Experiment 1, the clinical, hematological, biochemical and uterine histological characteristics of β-hemolytic and non-hemolyticE. coli pyometra bitches were compared. More (p < 0.05) metritis cases were observed in β-hemolyticE. coli pyometra uteri than in non-hemolyticE. coli pyometra uteri. β-hemolyticE. coli pyometra endometria had higher gene transcription ofIL -1β andIL -8 and lower gene transcription ofIL -6 than non-hemolyticE. coli pyometra endometria (p < 0.01). In Experiment 2, the immune response of endometrial epithelial and stromal cells, to hemolytic (Pyo18) and non-hemolyticE. coli strains (Pyo18 with deletedhlya -Pyo18ΔhlyA - and Pyo14) were compared. Following 4 h of incubation, Pyo18 decreased epithelial cell numbers to 54% (p < 0.001), and induced death of all stromal cells (p < 0.0001), whereas Pyo18ΔhlyA and Pyo14 had no effect on cell numbers. Compared to Pyo18ΔhlyA and Pyo14, respectively, Pyo18 induced a lower transcription level ofIL -1β (0.99 vs 152.0 vs 50.9 fold increase, p < 0.001), TNFα (3.2 vs 49.9 vs 12.9 fold increase, p < 0.05) andIL -10 (0.4 vs 3.6 vs 2.6 fold increase, p < 0.001) in stromal cells, after 1 h of incubation. This may be seen as an attempt of hemolyticE. coli to delay the activation of the immune response. In conclusion, endometrial epithelial and stromal cell damage induced by HlyA is a potential relevant step ofE. coli virulence in the pathogenesis of pyometra.