Reclassification of the taxonomic status of SEMIA3007 isolated in Mexico B-11A Mex as Rhizobium leguminosarum bv. viceae by bioinformatic tools. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Kishi, Luciano; Fernandes, Camila; Omori, Wellington; Campanharo, João; Lemos, Eliana
• Is Part Of: BMC microbiology. Volume 16:Issue 1(2016)
• Page Start: 1
• Page End: 8
• Publication Date: 2016-12
• Subjects: Genome sequencing -- Core genome -- Comparative analysis -- Ortholog genes -- Phylogenetic analysis; Microbiology -- Periodicals; 579.05
• DOI: 10.1186/s12866-016-0882-5
• Languages: English
• ISSNs: 1471-2180

Abstract

Abstract Background Evidence based on genomic sequences is extremely important to confirm the phylogenetic relationships within theRhizobium group. SEMIA3007 was analyzed within theMesorhizobium groups to define the underlying causes of taxonomic identification. We previously used biochemical tests and phenotypic taxonomic methods to identify bacteria, which can lead to erroneous classification. An improved understanding of bacterial strains such as theMesorhizobium genus would increase our knowledge of classification and evolution of these species. Results In this study, we sequenced the complete genome of SEMIA3007 and compared it with five otherMesorhizobium and twoRhizobium genomes. The genomes of isolated SEMIA3007 showed several orthologs withM. huakuii, M. erdmanii andM. loti . We identified SEMIA3007 as aMesorhizobium by comparing the 16S rRNA gene and the complete genome. Conclusion Our ortholog, 16S rRNA gene and average nucleotide identity values (ANI) analysis all demonstrate SEMIA3007 is notRhizobium leguminosarum bv. viceae . The results of the phylogenetic analysis clearly show SEMIA3007 is part of theMesorhizobium group and suggest a reclassification is warranted.