Anvillea garcinii extract inhibits the oxidative burst of primary human neutrophils. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Boukemara, Hanane; Hurtado-Nedelec, Margarita; Marzaioli, Viviana; Bendjeddou, Dalila; El Benna, Jamel; Marie, Jean-Claude
• Is Part Of: BMC complementary and alternative medicine. Volume 16:Issue 1(2016)
• Page Start: 1
• Page End: 10
• Publication Date: 2016-12
• Subjects: Inflammation -- NADPH oxidase -- Anvillea garcinii -- Zygophyllum gaetulum -- North Africa; Alternative medicine -- Periodicals; Complementary Therapies -- Periodicals; 615.505
• DOI: 10.1186/s12906-016-1411-7
• Languages: English
• ISSNs: 1472-6882

Abstract

Abstract Background Anvillea garcinii Coss. & Durieu (Anv ) plant is used as a traditional North African medicine against several diseases associated with inflammation. At inflammatory sites, reactive oxygen species (ROS) produced in excess by activated phagocyte NADPH oxidase (NOX2) can accentuate inflammatory responses. Thus, we investigated ifAnv -water soluble polysaccharides could modulate primary human neutrophil oxidative burst in vitro. Methods Human neutrophils were isolated from fresh whole blood and O2 .- generation was measured by cytochromec reduction assays. Western blots were used to analyse the translocation of PKC, p47phox (a key component of NOX2 activity) to neutrophil plasma membrane. Also, myeloperoxidase (MPO) release in the extracellular medium was studied by western blots. Flow cytometric analysis was used to detect CD11b membrane expression. Results Water soluble polysaccharides fromAnv dose-dependently inhibited N-formyl-methionyl-leucyl-phenylalanine (fMLF)- and phorbol myristate acetate (PMA)-induced O2 .- generation by human neutrophils. Moreover, Anv -polysaccharides strongly inhibited PMA-induced PKCβ and p47phox translocation to membranes and p47phox phosphorylation on Ser328, a main PKC target. In contrast, polysaccharides extract fromZygophyllum gaetulum plant, which is also used as a traditional North African medicine against inflammatory diseases, was ineffective on this PKCβ-p47phox pathway. Further, Anv inhibited important neutrophil degranulation markers corresponding to myeloperoxidase (MPO) release and CD11b membrane expression. Conclusion The process of down-regulating NADPH oxidase by polysaccharides extracts fromAnv provides new insights into the mechanism ofAnv 's anti-inflammatory actions.