A case report of a cystic fibrosis patient with repeated isolation of Trichosporon mycotoxinivorans identified by a novel short-extraction method. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Goldenberger, Daniel; Hinić, Vladimira; Prince, Spasenija; Tamm, Michael; Balestra, Anna-Maria; Hohler, Doris; Frei, Reno
• Is Part Of: BMC infectious diseases. Volume 16:Issue 1(2016)
• Page Start: 1
• Page End: 5
• Publication Date: 2016-12
• Subjects: Trichosporon mycotoxinivorans -- Cystic fibrosis (CF) -- Internal transcribed spacer (ITS) -- Emerging pathogen -- MALDI-TOF MS; Communicable diseases -- Periodicals; Sexually Transmitted Diseases -- Periodicals; 616.905
• DOI: 10.1186/s12879-016-1910-7
• Languages: English
• ISSNs: 1471-2334

Abstract

Abstract Background Trichosporon mycotoxinivorans is a recently described yeast-like fungal organism and its association as a pathogen for patients with cystic fibrosis (CF) was reported previously. We show the clinical course of a CF patient over 9 years as well as the applications of modern molecular and proteomic identification techniques of this rare fungus. Case presentation We present the case of a 32-year-old male CF patient with sputum cultures continuously positive with the anamorphic yeastT. mycotoxinivorans during 9 years. Furthermore, susceptibility testing ofT. mycotoxinivorans to different antifungals were performed. In addition, a rapid identification method of this novel fungal pathogen with matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was applied using a simple extraction protocol. Conclusions Our case presentation confirmsT. mycotoxinivorans as a potential emerging pathogen in patients with CF. However, our CF patient showed mild symptoms over a very long time period of 9 years. A short MALDI-TOF MS procedure allows reliable and rapid identification ofT. mycotoxinivorans and therefore should facilitate further study on the clinical relevance and epidemiology of this unusual fungal organism.