Draft genome sequence of Streptomyces sp. TP-A0867, an alchivemycin producer. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Komaki, Hisayuki; Ichikawa, Natsuko; Oguchi, Akio; Hamada, Moriyuki; Harunari, Enjuro; Kodani, Shinya; Fujita, Nobuyuki; Igarashi, Yasuhiro
• Is Part Of: Standards in genomic sciences. Volume 11:Issue 1(2016)
• Page Start: 1
• Page End: 10
• Publication Date: 2016-12
• Subjects: Alchivemycin -- Biosynthetic gene cluster -- Genome mining -- Polyketide synthase -- Streptomyces -- Taxonomy; Genomics -- Periodicals; Genomics -- Periodicals; Genomics; Periodicals; 572.8605
• DOI: 10.1186/s40793-016-0207-1
• Languages: English
• ISSNs: 1944-3277

Abstract

Abstract Streptomyces sp. TP-A0867 (=NBRC 109436) produces structurally complex polyketides designated alchivemycins A and B. Here, we report the draft genome sequence of this strain together with features of the organism and assembly, annotation, and analysis of the genome sequence. The 9.9 Mb genome ofStreptomyces sp. TP-A0867 encodes 8, 385 putative ORFs, of which 7, 232 were assigned with COG categories. We successfully identified a hybrid polyketide synthase (PKS)/ nonribosomal peptide synthetase (NRPS) gene cluster that could be responsible for alchivemycin biosynthesis, and propose the biosynthetic pathway. The alchivemycin biosynthetic gene cluster is also present inStreptomyces rapamycinicus NRRL 5491T, Streptomyces hygroscopicus subsp. hygroscopicus NBRC 16556, andStreptomyces ascomycinicus NBRC 13981T, which are taxonomically highly close to strain TP-A0867. This study shows a representative example that distribution of secondary metabolite genes is correlated with evolution within the genusStreptomyces .