A cluster of KPC-2 and VIM-2-producing Klebsiella pneumoniae ST833 isolates from the pediatric service of a Venezuelan Hospital. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Falco, Aura; Ramos, Yusibeska; Franco, Esther; Guzmán, Alegría; Takiff, Howard
• Is Part Of: BMC infectious diseases. Volume 16:Issue 1(2016)
• Page Start: 1
• Page End: 9
• Publication Date: 2016-12
• Subjects: Klebsiella pneumoniae -- carbapenem -- resistance -- ST833; Communicable diseases -- Periodicals; Sexually Transmitted Diseases -- Periodicals; 616.905
• DOI: 10.1186/s12879-016-1927-y
• Languages: English
• ISSNs: 1471-2334

Abstract

Abstract Background Klebsiella pneumoniae is a bacterial pathogen that has developed resistance to multiple antibiotics and is a major cause of nosocomial infections worldwide. Carbapenemase-producingKlebsiella pneumoniae have been isolated in many hospitals in Venezuela, but they have not been well-studied. The aim of this study was to characterize carbapenem-resistantKlebsiella pneumoniae isolates from the pediatric service of a hospital located in Anzoategui State, in the eastern part of Venezuela. Methods NineteenKlebsiella pneumoniae strains isolated in the hospital from April to July 2014 were evaluated phenotypically and molecularly for the presence of carbapenemasesbla KPC, bla IMP andbla VIM. Molecular epidemiology was performed with Repetitive Extragenic Palindromic-PCR (REP-PCR) and Multilocus Sequence Typing (MLST). They were also studied for phenotypic and molecular resistance to a quaternary ammonium compound (QAC) disinfectant. Results All 19 isolates contained bothbla VIM-2 andbla KPC-2 genes, and thebla KPC-2 gene was associated withTn 4401b. All isolates were phenotypically sensitive to QACs and containedqac ΔE andadd A2 genes typical of class 1 integrons. Analysis by REP-PCR and MLST showed that all isolates had identical profiles characteristic of sequence type ST833. Conclusion All 19 strains arebla VIM-2 andbla KPC -2− producing ST833K. pneumoniae sensitive to QACs. This analysis may help to understand the routes of dissemination and confirms that QAC disinfectants can be used to help control their spread.