Monocyte chemoattractant protein-1 is not required for liver regeneration after partial hepatectomy. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Wyler, Stephanie; D'Ingillo, Shawna; Lamb, Cheri; Mitchell, Kristen
• Is Part Of: Journal of inflammation. Volume 13:Issue 1(2016)
• Page Start: 1
• Page End: 8
• Publication Date: 2016-12
• Subjects: MCP-1 -- CCR2 -- Macrophages -- Kupffer cells -- Partial hepatectomy -- Liver regeneration; Inflammation -- Periodicals; 616.047305
• DOI: 10.1186/s12950-016-0136-1
• Languages: English
• ISSNs: 1476-9255

Abstract

Abstract Background Liver regeneration following 70 % partial hepatectomy (PH) requires the coordinated expression of soluble mediators produced by macrophages. Monocyte chemoattractant protein-1 (MCP-1) is a potent stimulus of monocyte recruitment and macrophage activation. The goal of this study was to determine how MCP-1 contributes to liver regeneration. Methods PH was performed on anesthetized C57Bl/6 (wild type) and MCP-1 knockout mice, and macrophage-produced cytokines and hepatocyte proliferation were measured. Results In wild type mice, hepatic MCP-1 protein levels increased 4–6 h after PH, and elevated plasma MCP-1 levels were detected 12 h after PH. Hepatocyte proliferation was comparable in MCP-1 knockout and wild type mice, as was the expression of macrophage-derived cytokines, TNFα and IL-6, and levels of phosphorylated STAT3. The number of CCR2+ cells in the liver was similar in MCP-1 knockout and wild type mice, which suggests that other chemokines may recruit CCR2+ cells in the absence of MCP-1. Studies with CCR2 knockout mice revealed that hepatocyte proliferation was suppressed ~40 % compared to wild type mice 36 h after PH, but proliferation and liver-body-weight ratios were similar at 48 h. Conclusion These findings suggest that MCP-1 is not required for PH-induced liver regeneration, yet the role of CCR2 warrants further study.