Molecular identification of Mycobacterium bovis from cattle and human host in Mali: expanded genetic diversity. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Diallo, Mamadou; Diarra, Bassirou; Sanogo, Moumine; Togo, Antieme; Somboro, Anou; Diallo, Mariam; Traoré, Bréhima; Maiga, Mamoudou; Koné, Younoussa; Tounkara, Karim; Sarro, Yeya; Baya, Bocar; Goita, Drissa; Kassambara, Hamadoun; Dembélé, Bindongo; Siddiqui, Sophia; Murphy, Robert; Dao, Sounkalo; Diallo, Souleymane; Tounkara, Anatole; Niang, Mamadou
• Is Part Of: BMC veterinary research. Volume 12:Issue 1(2016)
• Page Start: 1
• Page End: 9
• Publication Date: 2016-12
• Subjects: Bovine tuberculosis -- Frequency -- Spoligotyping -- Mali; Veterinary medicine -- Research -- Periodicals; Veterinary medicine -- Periodicals; 636.0890724
• DOI: 10.1186/s12917-016-0768-7
• Languages: English
• ISSNs: 1746-6148

Abstract

Abstract Background Bovine tuberculosis (BTB) is a contagious, debilitating human and animal disease caused byMycobacterium bovis, a member of theMycobacterium tuberculosis complex. The study objective were to estimate the frequency of BTB, examine genetic diversity of theM. bovis population in cattle from five regions in Mali and to determine whetherM. bovis is involved in active tuberculosis (TB) in humans. Samples from suspected lesions on cattle at the slaughterhouses were collected. Mycobacterial smear, culture confirmation, and spoligotyping were used for diagnosis and species identification. Mycobacterium DNA from TB patients was spoligotyped to identifyM. bovis . Results In total, 675 cattle have been examined for lesions in the five regions of Mali. Out of 675 cattle, 79 specimens presented lesions and then examined for the presence ofM. bovis . Thus, 19 (24.1 %) were identified asM. bovis ; eight (10.1 %) were non-tuberculousMycobacterium (NTM). Nineteen spoligotype patterns were identified among 79 samples with five novel patterns. One case ofM. bovis (spoligotype pattern SB0300) was identified among 67 TB patients. Conclusion This study estimates a relatively true proportion of BTB in the regions of Mali and reveals new spoligotype patterns.