Cobalamin production by Lactobacillus coryniformis: biochemical identification of the synthetized corrinoid and genomic analysis of the biosynthetic cluster. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Torres, Andrea; Vannini, Verónica; Bonacina, Julieta; Font, Graciela; Saavedra, Lucila; Taranto, María
• Is Part Of: BMC microbiology. Volume 16:Issue 1(2016)
• Page Start: 1
• Page End: 9
• Publication Date: 2016-12
• Subjects: Lactobacillus -- Cobalamin production -- Coenzyme B12 gene cluster organization; Microbiology -- Periodicals; 579.05
• DOI: 10.1186/s12866-016-0854-9
• Languages: English
• ISSNs: 1471-2180

Abstract

Abstract Background Despite the fact that most vitamins are present in a variety of foods, malnutrition, unbalanced diets or insufficient intake of foods are still the cause of vitamin deficiencies in humans in some countries. Vitamin B12 (Cobalamin) is a complex compound that is only naturally produced by bacteria and archea. It has been reported that certain strains belonging to lactic acid bacteria group are capable of synthesized water-soluble vitamins such as those included in the B-group, as vitamin B12 . In this context, the goal of the present paper was to evaluate and characterize the production of vitamin B12 inLactobacillus coryniformis CRL 1001, a heterofermentative strain isolated from silage. Results Cell extract ofL. coryniformis CRL 1001, isolated from silage, is able to correct the coenzyme B12 requirement ofSalmonella enterica serovar Typhimurium AR 2680 in minimal medium. The chemical characterization of the corrinoid-like molecule isolated from CRL 1001 cell extract using HPLC and mass spectrometry is reported. The majority of the corrinoid produced by this strain has adenine like Coα-ligand instead 5, 6-dimethylbenzimidazole. Genomic studies revealed the presence of the complete machinery of the anaerobic biosynthesis pathway of coenzyme B12 . The detected genes encode all proteins for the corrin ring biosynthesis and for the binding of upper (β) and lower (α) ligands in one continuous stretch of the chromosome. Conclusions The results here described show for the first time thatL. coryniformis subsp.coryniformis CRL 1001 is able to produce pseudocobalamin containing adenine instead of 5, 6-dimethlbenzimidazole in the Coα-ligand. Genomic analysis allowed the identification and characterization of the completede novo biosynthetic pathway of the corrinoid produced by the CRL 1001 strain.