Application to Photocatalytic H2 Production of a Whole‐Cell Reaction by Recombinant Escherichia coli Cells Expressing [FeFe]‐Hydrogenase and Maturases Genes. Issue 28 (19th May 2016)

• Record Type: Journal Article
• Authors: Honda, Yuki; Hagiwara, Hidehisa; Ida, Shintaro; Ishihara, Tatsumi
• Is Part Of: Angewandte Chemie international edition. Volume 55:Issue 28(2016)
• Page Start: 8045
• Page End: 8048
• Publication Date: 2016-05-19
• Subjects: Escherichia coli -- [FeFe]-hydrogenase -- hydrogen -- photocatalysis -- whole-cell reactions; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201600177
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: A photocatalytic H2 production system using an inorganic–bio hybrid photocatalyst could contribute to the efficient utilization of solar energy, but would require the development of a new approach for preparing a H2 ‐forming biocatalyst. In the present study, we constructed a recombinant strain of Escherichia coli expressing the genes encoding the [FeFe]‐hydrogenase and relevant maturases from Clostridium acetobutylicum NBRC 13948 for use as a biocatalyst. We investigated the direct application of a whole‐cell of the recombinant E . coli . The combination of TiO2, methylviologen, and the recombinant E . coli formed H2 under light irradiation, demonstrating that whole cells of the recombinant E . coli could be employed for photocatalytic H2 production without any time‐consuming and costly manipulations (for example, enzyme purification). This is the first report of the direct application of a whole‐cell reaction of recombinant E . coli to photocatalytic H2 production. Abstract : An inorganic–bio hybrid photocatalyst for photocatalytic H2 production was developed. A biocatalyst involving whole‐cell recombinant E . coli cells expressing genes encoding [FeFe]‐hydrogenase and relevant maturases (rectangle) was combined with TiO2 (circle) via the methylviologen MV 2+ /MV +. couple.