Suppressive effects of dRYamides on feeding behavior of the blowfly, Phormia regina. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Maeda, Toru; Nakamura, Yuki; Shiotani, Hajime; Hojo, Masaru; Yoshii, Taishi; Ida, Takanori; Sato, Takahiro; Yoshida, Morikatsu; Miyazato, Mikiya; Kojima, Masayasu; Ozaki, Mamiko
• Is Part Of: Zoological letters. Volume 1:Issue 1(2015)
• Page Start: 1
• Page End: 10
• Publication Date: 2015-12
• Subjects: Bioactive peptide -- dRYamide -- Feeding regulation -- Taste response -- Fly; Zoology -- Periodicals; 590.5
• DOI: 10.1186/s40851-015-0034-z
• Languages: English
• ISSNs: 2056-306X

Abstract

Abstract Recently, dRYamides-1 and -2 have been identified as ligands of the neuropeptide Y-like receptor CG5811 inDrosophila melanogaster . It has also been reported in brief that injection of dRYamide-1suppresses the early feeding behavior called proboscis extension reflex (PER) in the blowflyPhormia regina . Immunohistochemical analyses by our group using anti-dRYamide-1 antiserum indicated symmetrical localization of 32 immunoreactive cells in the brain ofP. regina . In order to analyze the mechanism of feeding regulation, we further investigated the effects of dRYamide-1 and -2 on intake volume, PER exhibition, and activity of the sugar receptor neuron. After injection of dRYamide-1 or -2, flies showed little change in the intake volume of sucrose solution, but a significant depression of PER to sucrose. Injection of dRYamide-1 revealed a significant decrease in the responsiveness of the sugar receptor neuron, although the injection of dRYamide-2 did not. These results suggest that the dRYamide peptides decrease feeding motivation in flies, as evaluated by PER threshold, through a mechanism that partially involves desensitization of the sugar receptor neuron.