A matter of collection and detection for intraoperative and noninvasive near‐infrared fluorescence molecular imaging: To see or not to see?. Issue 2 (23rd January 2014)

• Record Type: Journal Article
• Authors: Zhu, Banghe; Rasmussen, John C.; Sevick‐Muraca, Eva M.
• Is Part Of: Medical physics. Volume 41:Issue 2(2014)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2014-01-23
• Subjects: Biomedical imaging -- Laser imaging -- Imaging detectors and sensors -- Contrast -- Noise; biological tissues -- biomedical optical imaging -- CCD image sensors -- dyes -- fluorescence -- light scattering -- medical image processing -- molecular biophysics -- phantoms; optical imaging -- molecular imaging -- phantom -- performance standard -- charge‐coupled camera (CCD); Organic dyes or closely‐related compounds for producing dyes; Mordants; Lakes -- Biological material, e.g. blood, urine; Haemocytometers -- Digital computing or data processing equipment or methods, specially adapted for specific applications -- Image data processing or generation, in general -- Charge coupled imagers -- Transforming light or analogous information into electric information; Charge coupled devices -- Medical imaging -- Fluorescence -- Near infrared imaging -- Cameras -- Image detection systems -- Image sensors -- Medical image contrast -- Tissue engineering -- Tissues; Medical physics -- Periodicals; Medical physics; Geneeskunde; Natuurkunde; Toepassingen; Biophysics; Periodicals; Periodicals; Electronic journals; 610.153
• DOI: 10.1118/1.4862514
• Languages: English
• ISSNs: 0094-2405

Abstract

Abstract : Purpose: : Although fluorescence molecular imaging is rapidly evolving as a new combinational drug/device technology platform for molecularly guided surgery and noninvasive imaging, there remains no performance standards for efficient translation of "first‐in‐humans" fluorescent imaging agents using these devices. Methods: : The authors employed a stable, solid phantom designed to exaggerate the confounding effects of tissue light scattering and to mimic low concentrations (nM–pM) of near‐infrared fluorescent dyes expected clinically for molecular imaging in order to evaluate and compare the commonly used charge coupled device (CCD) camera systems employed in preclinical studies and in human investigational studies. Results: : The results show that intensified CCD systems offer greater contrast with larger signal‐to‐noise ratios in comparison to their unintensified CCD systems operated at clinically reasonable, subsecond acquisition times. Conclusions: : Camera imaging performance could impact the success of future "first‐in‐humans" near‐infrared fluorescence imaging agent studies.