VCAM-1+ placenta chorionic villi-derived mesenchymal stem cells display potent pro-angiogenic activity. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Du, Wenjing; Li, Xue; Chi, Ying; Ma, Fengxia; Li, Zongjin; Yang, Shaoguang; Song, Baoquan; Cui, Junjie; Ma, Tao; Li, Juanjuan; Tian, Jianjian; Yang, Zhouxin; Feng, Xiaoming; Chen, Fang; Lu, Shihong; Liang, Lu; Han, Zhi-Bo; Han, Zhong-Chao
• Is Part Of: Stem cell research & therapy. Volume 7:Issue 1(2016)
• Page Start: 1
• Page End: 13
• Publication Date: 2016-12
• Subjects: Mesenchymal stem cells -- Placenta -- Angiogenesis -- Paracrine -- Vascular cell adhesion molecule-1 (CD106); Stem cells -- Research -- Periodicals; Cellular therapy -- Periodicals; 616.0277405
• DOI: 10.1186/s13287-016-0297-0
• Languages: English
• ISSNs: 1757-6512

Abstract

Abstract Introduction Mesenchymal stem cells (MSCs) represent a heterogeneous cell population that is promising for regenerative medicine. The present study was designed to assess whether VCAM-1 can be used as a marker of MSC subpopulation with superior angiogenic potential. Methods MSCs were isolated from placenta chorionic villi (CV). The VCAM-1+/− CV-MSCs population were separated by Flow Cytometry and subjected to a comparative analysis for their angiogenic properties including angiogenic genes expression, vasculo-angiogenic abilities on Matrigelin vitro and in vivo, angiogenic paracrine activities, cytokine array, and therapeutic angiogenesis in vascular ischemic diseases. Results Angiogenic genes, including HGF, ANG, IL8, IL6, VEGF-A, TGFβ, MMP2 and bFGF, were up-regulated in VCAM-1+ CV-MSCs. Consistently, angiogenic cytokines especially HGF, IL8, angiogenin, angiopoitin-2, μPAR, CXCL1, IL-1β, IL-1α, CSF2, CSF3, MCP-3, CTACK, and OPG were found to be significantly increased in VCAM-1+ CV-MSCs. Moreover, VCAM-1+ CV-MSCs showed remarkable vasculo-angiogenic abilities by angiogenesis analysis with Matrigelin vitro and in vivo and the conditioned medium of VCAM-1+ CV-MSCs exerted markedly pro-proliferative and pro-migratory effects on endothelial cells compared to VCAM-1− CV-MSCs. Finally, transplantation of VCAM-1+ CV-MSCs into the ischemic hind limb of BALB/c nude mice resulted in a significantly functional improvement in comparison with VCAM-1− CV-MSCs transplantation. Conclusions VCAM-1+ CV-MSCs possessed a favorable angiogenic paracrine activity and displayed therapeutic efficacy on hindlimb ischemia. Our results suggested that VCAM-1+ CV-MSCs may represent an important subpopulation of MSC for efficient therapeutic angiogenesis.