Gallic acid improves endothelium‐dependent vasodilatory response to histamine in the mesenteric vascular bed of diabetic rats: 没食子酸可改善糖尿病大鼠肠系膜血管床对组胺的内皮依赖性血管舒张反应. (10th January 2017)

• Record Type: Journal Article
• Authors: Badavi, Mohammad; Bazaz, Amir; Dianat, Mahin; Sarkaki, Alireza
• Is Part Of: Journal of diabetes. Volume 9:Number 11(2017)
• Page Start: 1003
• Page End: 1011
• Publication Date: 2017-01-10
• Subjects: diabetes mellitus -- endothelium -- gallic acid -- histamine -- mesenteric arteries; 糖尿病 -- 内皮 -- 没食子酸 -- 组胺 -- 肠系膜动脉; Diabetes -- Periodicals; 618.3646005
• DOI: 10.1111/1753-0407.12513
• Languages: English
• ISSNs: 1753-0393

Abstract

Abstract: Background: Endothelial dysfunction is one of the many complications caused by diabetes mellitus. The aim of the present study was to evaluate the effects of gallic acid (GA) on the mesenteric vascular bed (MVB) response to histamine in diabetic rats. Methods: Forty male Wistar rats were randomly assigned to a control group, an untreated alloxan‐induced diabetic group and three diabetic groups treated with different doses of GA. Six weeks after induction of diabetes and GA treatment, total antioxidant capacity (TAC), malondialdehyde (MDA) concentrations, and the vasodilatory response to histamine of the MVB (measured as changes in perfusion pressure) were determined. Results: The vasodilatory response to histamine and TAC decreased, whereas MDA increased in the plasma from diabetic rats ( P < 0.01). However, in the presence of 3 × 10 −5 mol/L N G ‐nitro‐l‐ arginine methyl ester (a nitric oxide synthase inhibitor) and 1 × 10 −5 mol/L indomethacin (an inhibitor of prostaglandin production), the vasodilatory response of the MVB to histamine was reduced in all groups ( P < 0.001). Treatment of diabetic rats with 20 and 40 mg/kg per day GA, but not 10 mg/kg per day GA, increased TAC and decreased MDA concentrations ( P < 0.01 and P < 0.001 vs untreated diabetic rats, respectively) and significantly improved the vasodilatory response to histamine ( P < 0.05 and P < 0.001, respectively). Conclusion: The results show that, in diabetic rats, the endothelium‐dependent vasodilatory response of the MVB to histamine is significantly decreased and depends on both nitric oxide‐ and prostaglandin‐producing pathways and may be mediated by oxidative stress. Treatment with the antioxidant GA restored the vasodilatory response of the MVB to histamine. Abstract : Highlights This study shows that the vasodilatory response to histamine depends on both nitric oxide‐ and prostaglandin‐producing pathways and that diabetes‐induced changes are related more to altered cyclo‐oxygenase activity, which may be mediated by oxidative stress. As an antioxidant, gallic acid restored the vasodilatory response to histamine in diabetic rats. Therefore, gallic acid could have a protective role against diabetes‐induced oxidative stress and thereby improve endothelial dysfunction and vascular responses to histamine.