The SAV1322 gene from Staphylococcus aureus: genomic and proteomic approaches to identification and characterization of gene function. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Kim, Jung; Kim, Hyun-Kyung; Kang, Gi; Kim, Il-Hwan; Kim, Hwa; Lee, Yeong; Yoo, Jae
• Is Part Of: BMC microbiology. Volume 16:Issue 1(2016)
• Page Start: 1
• Page End: 12
• Publication Date: 2016-12
• Subjects: Heat shock proteins -- Microarray analysis -- Microbial drug resistance -- Physiological stress -- Proteomics -- Staphylococcus aureus; Microbiology -- Periodicals; 579.05
• DOI: 10.1186/s12866-016-0824-2
• Languages: English
• ISSNs: 1471-2180

Abstract

Abstract Background Bacterial two-component regulatory systems (TCRS) are associated with the expression of virulence factors and antibiotic susceptibility. InStaphylococcus aureus, 16 TCRS types have been identified. The histidine kinase/response regulator SAV1321/SAV1322 in theS. aureus shares considerable homology with the TCRS DesKR inBacillus subtilis . However, a function for the SAV1322 locus has not yet been assigned. Results Deletion of the SAV1322 locus inS. aureus results in reduced growth when cultured under low (25 °C) and high (46 °C) temperature conditions. Thesav1322 deletion mutant is more tolerant to oxidative stress in vitro and is less pathogenic in a murine infection model when compared with wild-type parent strain Mu50. Furthermore, thesav1322 mutant exhibits lower MICs for gentimicin, tetracyclines and glycopeptides, increased autolysis, and a thinner cell wall when compared with the wild-type strain. Microarray and proteomic analyses show that the expression of cell-wall-associated genesglmS andmurZ are lower, and the expression of heat shock and stress-related genes (hrcA, ctsR, dnaK, dnaJ, grpE, clpB, andclpC ) are higher in thesav1322 mutant when compared with the wild-type strain. In addition, thesav1322 mutant displays altered expression of proteins involved in carbohydrate/energy metabolism, cell wall metabolism, and stress or heat shock response, as well as other metabolic processes including lipid metabolism, amino acid biosynthesis, purine or pyrimidine metabolism, transcription, and protein biosynthesis. Conclusions TheS. aureus SAV1322 locus plays a pronounced role in temperature adaptation, antibiotic resistance, and virulence by regulating a wide range of genes and proteins involved in metabolism and stress tolerance.