Genotypic and phenotypic relatedness of Pseudomonas aeruginosa isolates among the major cystic fibrosis patient cohort in Italy. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Cigana, Cristina; Melotti, Paola; Baldan, Rossella; Pedretti, Elisa; Pintani, Emily; Iansa, Patrizia; De Fino, Ida; Favari, Flavio; Bergamini, Gabriella; Tridello, Gloria; Cirillo, Daniela; Assael, Baroukh; Bragonzi, Alessandra
• Is Part Of: BMC microbiology. Volume 16:Issue 1(2016)
• Page Start: 1
• Page End: 8
• Publication Date: 2016-12
• Subjects: Cystic fibrosis -- Pseudomonas aeruginosa -- Bacterial genotype -- Bacterial phenotype -- Bacterial adaptation; Microbiology -- Periodicals; 579.05
• DOI: 10.1186/s12866-016-0760-1
• Languages: English
• ISSNs: 1471-2180

Abstract

Abstract Background Pseudomonas aeruginosa is the predominant pathogen associated with the decline of pulmonary function in cystic fibrosis (CF) patients. Both environment-to-host acquisition and patient-to-patient transmission have been described forP. aeruginosa infection. Epidemic clones and bacterial phenotypic adaptation to the CF lung have been recognised as independent risk factors for disease progression. So far, there is no established link between genotypic prevalence and phenotypic traits. Here, we look at the major CF patient cohort in Italy to identify sharedP. aeruginosa clones and associated common phenotypic traits. Results A comprehensive analysis ofP. aeruginosa genotypes to determine the presence of high-risk shared clones and their association to specific phenotypic traits has been performed in a major Italian CF centre. Pulsed-field gel electrophoresis (PFGE) ofP. aeruginosa isolates from 338 CF subjects identified 43 profiles shared by two or more patients and 214 profiles exclusive to individual patients. There was no evidence of aP. aeruginosa outbreak, but four most prevalent pulsotypes were detected. Common phenotypic traits were recorded intra-pulsotypes, but we detected heterogeneity inter-pulsotypes. Two of the four major pulsotypes includedP. aeruginosa isolates with hallmarks of adaptation to the CF airways, including loss of motility, low production of siderophore, pyocyanin and proteases, and antibiotic resistance. One of these pulsotypes grouped a high percentage of hypermutable isolates. No clear correlation between epidemiological and clinical data was found. Conclusions We conclude that CF patients of this cohort shared common pulsotypes, but their phenotypic heterogeneity indicates an absence of specific traits associated toP. aeruginosa genotypic prevalence.