Convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Bartosik, Aneta; Glabski, Krzysztof; Kulinska, Anna; Lewicka, Ewa; Godziszewska, Jolanta; Markowska, Aleksandra; Jagura-Burdzy, Grazyna
• Is Part Of: BMC microbiology. Volume 16:Issue 1(2016)
• Page Start: 1
• Page End: 15
• Publication Date: 2016-12
• Subjects: Broad-host-range -- Cloning vector -- RK2 -- lacZ -- xylE reporter -- Stability functions; Microbiology -- Periodicals; 579.05
• DOI: 10.1186/s12866-016-0674-y
• Languages: English
• ISSNs: 1471-2180

Abstract

Abstract Background Low-copy-number vectors of potential wide application in biotechnology need to encode stabilization modules ensuring their stable inheritance. The efficiency of stabilization may vary depending on the plasmid host so a thorough analysis of stabilization functions is required before use. Results To facilitate such analysis highly unstable, mobilizable, broad-host-range (BHR) vectors based on RK2 replicon were constructed. The vectors are suitable for testing of various stabilization functions, including plasmid and chromosomal partitioning cassettes encoding ParB homologues capable of spreading on DNA. ThexylE orlacZ reporter systems facilitate easy monitoring of plasmid segregation. Conclusion The range of BHR vectors with different reporter cassettes and alternative mobilization systems expands their application in diverse bacterial species.