Improvement of pea biomass and seed productivity by simultaneous increase of phloem and embryo loading with amino acids. (3rd December 2014)

• Record Type: Journal Article
• Authors: Zhang, Lizhi; Garneau, Matthew G.; Majumdar, Rajtilak; Grant, Jan; Tegeder, Mechthild
• Is Part Of: Plant journal. Volume 81:Number 1(2015:Jan.)
• Page Start: 134
• Page End: 146
• Publication Date: 2014-12-03
• Subjects: biomass production -- nitrogen and carbon metabolism -- phloem and seed loading -- seed yield -- sink development -- source to sink amino acid transport; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.12716
• Languages: English
• ISSNs: 0960-7412

Abstract

Summary: The development of sink organs such as fruits and seeds strongly depends on the amount of nitrogen that is moved within the phloem from photosynthetic‐active source leaves to the reproductive sinks. In many plant species nitrogen is transported as amino acids. In pea ( Pisum sativum L.), source to sink partitioning of amino acids requires at least two active transport events mediated by plasma membrane‐localized proteins, and these are: (i) amino acid phloem loading; and (ii) import of amino acids into the seed cotyledons via epidermal transfer cells. As each of these transport steps might potentially be limiting to efficient nitrogen delivery to the pea embryo, we manipulated both simultaneously. Additional copies of the pea amino acid permease PsAAP1 were introduced into the pea genome and expression of the transporter was targeted to the sieve element‐companion cell complexes of the leaf phloem and to the epidermis of the seed cotyledons. The transgenic pea plants showed increased phloem loading and embryo loading of amino acids resulting in improved long distance transport of nitrogen, sink development and seed protein accumulation. Analyses of root and leaf tissues further revealed that genetic manipulation positively affected root nitrogen uptake, as well as primary source and sink metabolism. Overall, the results suggest that amino acid phloem loading exerts regulatory control over pea biomass production and seed yield, and that import of amino acids into the cotyledons limits seed protein levels. Significance Statement: Using Pisum sativum L. as model this work provides strong evidence that both phloem and embryo loading of amino acids are essential for efficient source to sink transport of nitrogen. We demonstrate that amino acid phloem loading exerts regulatory control over pea biomass production and seed yield, and that import of amino acids into the cotyledons limits seed protein levels.