Six‐gene Assay as a new biomarker in the blood of patients with colorectal cancer: establishment and clinical validation. Issue 4 (18th February 2019)

• Record Type: Journal Article
• Authors: Shou, Xin; Li, Yong; Hu, Weilei; Ye, Tingting; Wang, Guosheng; Xu, Feng; Sui, Meihua; Xu, Yibing
• Is Part Of: Molecular oncology. Volume 13:Issue 4(2019)
• Page Start: 781
• Page End: 791
• Publication Date: 2019-02-18
• Subjects: biomarker -- circulating tumor cells -- colorectal cancer -- RT‐PCR -- Six‐gene Assay; Cancer -- Molecular aspects -- Periodicals; 616.994005
• DOI: 10.1002/1878-0261.12427
• Languages: English
• ISSNs: 1574-7891

Abstract

Abstract : Colorectal cancer (CRC) is the second most common cancer in men and the third most common cancer in women. Although long‐term survival has improved over the past 30 years, at least 50% of patients with CRC will develop metastases after diagnosis. In this study, we examined whether quantifying the mRNA of six CRC‐related genes in the blood could improve disease assessment through detection of circulating tumor cells (CTC), and thereby improve progression prediction in relapsed CRC patients. Cell spiking assay and RT‐PCR were performed with blood samples from healthy volunteers spiked with six CRC cell lines to generate an algorithm, herein called the Six‐gene Assay, based on six genes ( CEA, EpCAM, CK19, MUC1, EGFR and C‐Met ) for CTC detection. The CTCs of 50 relapsed CRC patients were then respectively measured by CEA Gene Assay (single‐gene assay control) and Six‐gene Assay. Subsequently, receiver operating characteristic analysis of the CTC panel performance in diagnosing CRC was conducted for both assays. Moreover, the 2‐year progression‐free survival (PFS) of all patients was collected, and the application of CEA Gene Assay and Six‐gene Assay in predicting PFS was carefully evaluated with different CTC cutoff values. Encouragingly, we successfully constructed the first multiple gene‐based algorithm, named the Six‐gene Assay, for CTC detection in CRC patients. Six‐gene Assay was more sensitive than CEA Gene Assay; for instance, in 50 CRC patients, the positive rate of Six‐gene Assay in CTC detection was 82%, whereas that of CEA Gene Assay was only 70%. Moreover, Six‐gene Assay was more sensitive and accurate than CEA Gene Assay in diagnosing CRC as well as predicting the 2‐year PFS of CRC patients. Statistical analysis demonstrated that CTC numbers measured by Six‐gene Assay were significantly associated with 2‐year PFS. This novel Six‐gene Assay improves the definition of disease status and correlates with PFS in relapsed CRC, and thus holds promise for future clinical applications. Abstract : Based on the mRNA expression of CEA, EpCAM, CK19, MUC1, EGFR and C‐Met, we constructed the first multiple gene‐based algorithm, called the Six‐gene Assay, for detection of circulating tumor cells (CTC) in colorectal cancer (CRC) patients. Clinical validation of Six‐gene Assay demonstrated its superiority over CEA Gene Assay in disease assessment and progression prediction in relapsed CRC. Six‐gene Assay holds promise for future clinical applications.