Simple and sensitive monitoring of β2-agonist residues in meat by liquid chromatography–tandem mass spectrometry using a QuEChERS with preconcentration as the sample treatment. (July 2015)

• Record Type: Journal Article
• Authors: Xiong, Lin; Gao, Ya-Qin; Li, Wei-Hong; Yang, Xiao-Lin; Shimo, Shimo Peter
• Is Part Of: Meat science. Volume 105(2015:Jul.)
• Page Start: 96
• Page End: 107
• Publication Date: 2015-07
• Subjects: Cimaterol (PubChem CID: 277) -- Terbutaline (PubChem CID: 5403) -- Salbutamol (PubChem CID: 2083) -- Fenoterol (PubChem CID: 3343) -- Ractopamine (PubChem CID: 56052) -- Clorprenaline (PubChem CID: 23360) -- Clenbuterol (PubChem CID: 2783) -- Tulobuterol (PubChem CID: 5606) -- Phenylethanolamine A (PubChem CID: 3039588) -- Penbuterol (PubChem CID: 4845); QuEChERS -- β2-Agonists -- LC–MS/MS -- Feed additive -- Meat -- DVB–NVP–SO3Na; Meat -- Periodicals; Meat industry and trade -- Periodicals; Viande -- Périodiques; Viande -- Industrie -- Périodiques; Meat; Meat industry and trade; Periodicals; 641.36
• DOI: 10.1016/j.meatsci.2015.03.013
• Languages: English
• ISSNs: 0309-1740

Abstract

Abstract: A liquid chromatography with tandem mass spectrometric detection (LC–MS/MS) method was established for the simultaneous determination of the levels of 10 β2 -agonists in meat. The samples were extracted using an aqueous acidic solution and cleaned up using a Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) technique utilising a DVB–NVP–SO3 Na sorbent synthesised in-house. First, the β2 -agonist residues were extracted in an aqueous acidic solution, followed by matrix solid-phase dispersion for clean-up. The linearities of the method were R 2 = 0.9925–0.9998, with RSDs of 2.7–15.3% and 73.7–103.5% recoveries. Very low limits of detection (LOD) and quantitation (LOQ) of 0.2–0.9 μg/kg and 0.8–3.2 μg/kg, respectively, were achieved for spiked meat. The values obtained were lower than the maximum residue limits (MRLs) established by the EU and China. These results clearly demonstrate the feasibility of the proposed approach. The evaluated method provided reliable screening, quantification and identification of 10 β2 -agonists in meat. Highlights: Development of new rapid analytical method for 10 β2 -agonists in meat at levels of trace amounts Combination QuEChERS–LC–MS/MS was applied. DVB–NVP–SO3 Na was synthesised and firstly applied it as the cleansing agent in QuEChERS method. The method's advantages are rapider, cheaper, excellent recovery and RSDs, and very low LOD and LOQ. The processes of extraction and clean are environmentally friendly.