Detection of specific DNA sequences in Maize (Zea mays L.) based on phosphorescent quantum-dot exciton energy transfer. (11th March 2019)
- Record Type:
- Journal Article
- Title:
- Detection of specific DNA sequences in Maize (Zea mays L.) based on phosphorescent quantum-dot exciton energy transfer. (11th March 2019)
- Main Title:
- Detection of specific DNA sequences in Maize (Zea mays L.) based on phosphorescent quantum-dot exciton energy transfer
- Authors:
- Lv, Jinzhi
Miao, Yanming
Yan, Guiqin - Abstract:
- Abstract : The complementary sequence of genetically-modified marker sequence cauliflower mosaic virus 35S promoter (Ca MV 35S) DNA was trimmed and designed into sequences S1 and S2, which were separately modified onto the surfaces of room-temperature phosphorescent (RTP) quantum dots (QDs), forming QDs-S1 (P1) and QDs-S2 (P2), respectively. Abstract : The complementary sequence of genetically-modified marker sequence cauliflower mosaic virus 35S promoter (Ca MV 35S) DNA was trimmed and designed into sequences S1 and S2, which were separately modified onto the surfaces of room-temperature phosphorescent (RTP) quantum dots (QDs), forming QDs-S1 (P1) and QDs-S2 (P2), respectively. P1 and P2 were used as phosphorescent responsive units and identifying units. The target DNA sequence was added to induce P1 and P2 assembly aggregation, and the phosphorescence was self-quenched. Also, with the reduction of phosphorescence lifetime, a novel phosphorescent exciton energy transfer nano-probe system was built, which was used to quantitatively detect the genetically modified marker sequence Ca MV 35S in Maize ( Zea mays L.). This simple and marker-free biological sensing method had a linear range of 10–200 nM for detecting the target DNA sequence ( R = 0.991) and a detection limit (3 σ ) of 0.48 nM. Owing to the phosphorescence properties of QDs, this method is not affected by the background fluorescence and scattering light in biological fluids, and it is very feasible for theAbstract : The complementary sequence of genetically-modified marker sequence cauliflower mosaic virus 35S promoter (Ca MV 35S) DNA was trimmed and designed into sequences S1 and S2, which were separately modified onto the surfaces of room-temperature phosphorescent (RTP) quantum dots (QDs), forming QDs-S1 (P1) and QDs-S2 (P2), respectively. Abstract : The complementary sequence of genetically-modified marker sequence cauliflower mosaic virus 35S promoter (Ca MV 35S) DNA was trimmed and designed into sequences S1 and S2, which were separately modified onto the surfaces of room-temperature phosphorescent (RTP) quantum dots (QDs), forming QDs-S1 (P1) and QDs-S2 (P2), respectively. P1 and P2 were used as phosphorescent responsive units and identifying units. The target DNA sequence was added to induce P1 and P2 assembly aggregation, and the phosphorescence was self-quenched. Also, with the reduction of phosphorescence lifetime, a novel phosphorescent exciton energy transfer nano-probe system was built, which was used to quantitatively detect the genetically modified marker sequence Ca MV 35S in Maize ( Zea mays L.). This simple and marker-free biological sensing method had a linear range of 10–200 nM for detecting the target DNA sequence ( R = 0.991) and a detection limit (3 σ ) of 0.48 nM. Owing to the phosphorescence properties of QDs, this method is not affected by the background fluorescence and scattering light in biological fluids, and it is very feasible for the recognition and detection of genetically-modified marker sequences in biological samples. … (more)
- Is Part Of:
- New journal of chemistry. Volume 43:Number 14(2019)
- Journal:
- New journal of chemistry
- Issue:
- Volume 43:Number 14(2019)
- Issue Display:
- Volume 43, Issue 14 (2019)
- Year:
- 2019
- Volume:
- 43
- Issue:
- 14
- Issue Sort Value:
- 2019-0043-0014-0000
- Page Start:
- 5308
- Page End:
- 5314
- Publication Date:
- 2019-03-11
- Subjects:
- Chemistry -- Periodicals
Chimie -- Périodiques
540 - Journal URLs:
- http://www.rsc.org/ ↗
http://www.rsc.org/is/journals/current/newjchem/njc.htm ↗ - DOI:
- 10.1039/c8nj06106h ↗
- Languages:
- English
- ISSNs:
- 1144-0546
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 6084.319900
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 9910.xml