Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Kasabova, Mariana; Villeret, Bérengère; Gombault, Aurélie; Lecaille, Fabien; Reinheckel, Thomas; Marchand-Adam, Sylvain; Couillin, Isabelle; Lalmanach, Gilles
• Is Part Of: Respiratory research. Volume 17:Issue 1(2016)
• Page Start: 1
• Page End: 5
• Publication Date: 2016-12
• Subjects: Bronchoalveolar lavage fluid -- Bleomycin -- Cathepsins -- Cystatins -- Fibrosis -- Lung -- Proteases; Respiratory organs -- Diseases -- Periodicals; 616.2005
• DOI: 10.1186/s12931-016-0432-6
• Languages: English
• ISSNs: 1465-993X

Abstract

Abstract The activity of cysteine cathepsin B increased markedly in lung homogenates and in bronchoalveolar lavage fluids (BALF) of the mouse model of bleomycin-induced lung fibrosis after 14 days of challenge. In contrast the level of the cysteine cathepsin inhibitor cystatin C was unaffected in BALF of wild-type and cathepsin B-deficient mice. Therefore, murine cystatin C is not a reliable marker of fibrosis during bleomycin-induced lung fibrosis. Current data are in sharp contrast to previous analysis carried on human BALF from patients with idiopathic pulmonary fibrosis, for which the level of cathepsin B remained unchanged while cystatin C was significantly increased.