Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Sliepen, Kwinten; Ozorowski, Gabriel; Burger, Judith; Montfort, Thijs; Stunnenberg, Melissa; LaBranche, Celia; Montefiori, David; Moore, John; Ward, Andrew; Sanders, Rogier
• Is Part Of: Retrovirology. Volume 12:Issue 1(2015)
• Page Start: 1
• Page End: 5
• Publication Date: 2015-12
• Subjects: HIV-1 -- Envelope glycoprotein -- Ferritin -- Nanoparticles -- Vaccine -- SOSIP -- BG505; Retroviruses -- Periodicals; 579.2569
• DOI: 10.1186/s12977-015-0210-4
• Languages: English
• ISSNs: 1742-4690

Abstract

Abstract Background Presenting vaccine antigens in particulate form can improve their immunogenicity by enhancing B cell activation. Findings We describe ferritin-based protein nanoparticles that display multiple copies of native-like HIV-1 envelope glycoprotein trimers (BG505 SOSIP.664). Trimer-bearing nanoparticles were significantly more immunogenic than trimers in both mice and rabbits. Furthermore, rabbits immunized with the trimer-bearing nanoparticles induced significantly higher neutralizing antibody responses against most tier 1A viruses, and higher responses (but not significantly), to several tier 1B viruses and the autologous tier 2 virus than when the same trimers were delivered as soluble proteins. Conclusions This or other nanoparticle designs may be practical ways to improve the immunogenicity of envelope glycoprotein trimers.