A two-photon fluorescent probe for basal formaldehyde imaging in zebrafish and visualization of mitochondrial damage induced by FA stress. Issue 7 (12th December 2018)

• Record Type: Journal Article
• Authors: Xin, Fangyun; Tian, Yong; Gao, Congcong; Guo, Bingpeng; Wu, Yulong; Zhao, Junfang; Jing, Jing; Zhang, Xiaoling
• Is Part Of: Analyst. Volume 144:Issue 7(2019)
• Page Start: 2297
• Page End: 2303
• Publication Date: 2018-12-12
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an02108b
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A two-photon fluorescence probeMito-FA-FP can monitor mitochondrial morphology change and image endogenous FA in vivo . Abstract : The detection of mitochondrial formaldehyde (FA) is of great significance because FA is generated through a one-carbon formaldehyde cycle in mitochondria, and abnormal elevations in FA levels can damage mitochondria by decreasing the mitochondrial membrane potential and inhibiting mitochondrial respiration. Herein, a mitochondria-targetable two-photon probe (Mito-FA-FP ) has been well demonstrated.Mito-FA-FP is conjugated with hydrazine as the FA-reactive site and a pyridine derivate as the mitochondria-targetable moiety. After reacting with FA, Mito-FA-FP exhibits dramatic fluorescence enhancement (12-fold) due to suppression of the PET process in the probe and presents good selectivity as well as high sensitivity (LOD: 12.4 μM). Moreover, Mito-FA-FP can be utilized to monitor endogenous FA in mitochondria and evaluate mitochondrial damage caused by FA stress through observing mitochondrial morphology changes. With good two-photon absorption cross-section and high two-photon fluorescence contrast, Mito-FA-FP has been successfully employed for the two-photon fluorescence imaging of basal FA in zebrafish.