Pectins from the pulp of gabiroba (Campomanesia xanthocarpa Berg): Structural characterization and rheological behavior. (15th June 2019)

• Record Type: Journal Article
• Authors: Barbieri, Shayla Fernanda; da Costa Amaral, Sarah; Ruthes, Andrea Caroline; de Oliveira Petkowicz, Carmen Lúcia; Kerkhoven, Nicole Cristine; da Silva, Elisangela Rodrigues Assunção; Silveira, Joana Léa Meira
• Is Part Of: Carbohydrate polymers. Volume 214(2019)
• Page Start: 250
• Page End: 258
• Publication Date: 2019-06-15
• Subjects: Myrtaceae family -- Pectin -- NMR analysis -- Rheology -- HPSEC-MALLS-RI; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.03.045
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A HM pectin from the gabiroba was obtained by an eco-friendly method. HG and RG-I were isolated and characterized for the first time from gabiroba pulp. A homogeneous pectic fraction (GWP-TEP) with 68.1% of GalA was obtained. 5% GW pectin had same shear thinning behavior in water and in the 0.1 mol L −1 NaCl. 5% GW pectin showed a gel behavior by dynamic rheological measurements. Abstract: The pulp of gabiroba fruits was submitted to a hot water extraction, giving rise to a crude pectin named GW. GW was shown to be composed mainly of arabinose (54.5%), galacturonic acid (33.5%), galactose (7.6%), and rhamnose (1.6%). GW was characterized by chromatographic and spectroscopic methods indicating the presence of homogalacturonans (HG) with a degree of methyl-esterification (DM) of 60% and rhamnogalacturonans I (RG-I). HG domain represents 31.9% and RG-I domain 65.3%. Furthermore, GW was submitted to sequential fractionation methods, giving rise to GWP-TEP fraction, structurally characterized by the predominance of HG regions, and confirmed by NMR analysis. The rheological behavior of GW was analyzed at 1%, 3%, and 5% (w/v) concentration with 0.1 mol L −1 NaCl. All samples showed shear thinning behavior. In the oscillatory measurements, the 1% GW showed a liquid-like behavior, while the 3% presented a concentrated solution behavior and the 5% GW a gel behavior.