A polysaccharide from dried aerial parts of Agrimonia pilosa: Structural characterization and its potential therapeutic activity for steroid‑induced necrosis of the femoral head (SANFH). (15th June 2019)

• Record Type: Journal Article
• Authors: Huang, Wei; Deng, Huan; Jin, Shengyang; Yang, Wenbo; Wang, Haili; Meng, Chunqing; Wang, Hong; Yang, Shuhua
• Is Part Of: Carbohydrate polymers. Volume 214(2019)
• Page Start: 71
• Page End: 79
• Publication Date: 2019-06-15
• Subjects: Agrimonia pilosa -- Polysaccharide -- Steroid induced avascular necrosis of the femoral head (SANFH) -- Osteoblast -- Apoptosis -- Dexamethasone; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.03.004
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A polysaccharide (APP-AW) was isolated and purified from Agrimonia pilosa. The structure of APP-AW was identified to be (1 → 3)-β-d -glucan. Pretreatment of APP-AW protects dexamethasone (Dex)-induced apoptosis in osteoblasts. APP-AW might be used to treat steroid‑induced necrosis of the femoral head (SANFH). Abstract: In this study, one homogeneous polysaccharide (APP-AW), with an average molecular weight of 9550 Da, was purified from Agrimonia pilosa . Analysis by gas chromatography (GC), methylation, UV, Infrared spectra (IR), 1D and 2D nuclear magnetic resonance (NMR) spectroscopy indicated that APP-AW was a β-(1 → 3)-d -glucan. The effect of APP-AW on dexamethasone (Dex)-induced apoptosis in osteoblasts was also examined. Pretreatment of APP-AW (100 μg/ml) significantly attenuated cell loss and apoptosis induced by Dex (1 μM) in osteoblasts as determined by MTT, Annexin V-FITC/ propidium iodide (PI) and Transferase (TdT)-mediated dUTP nick end labeling (TUNEL) staining assay. Collectively, the present study demonstrated that APP-AW might be an alternative therapeutics for the treatment of SANFH via reducing Dex‑induced bone cellular apoptosis.