Radiation treatment monitoring using multimodal functional imaging: PET/CT (18F-Fluoromisonidazole & 18F-Fluorocholine) and DCE-US. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Arteaga-Marrero, Natalia; Brekke Rygh, Cecilie; Mainou-Gomez, Jose; Adamsen, Tom; Lutay, Nataliya; Reed, Rolf; Olsen, Dag
• Is Part Of: Journal of translational medicine. Volume 13:Issue 1(2015)
• Page Start: 1
• Page End: 10
• Publication Date: 2015-12
• Subjects: PET/CT -- 18F-Fluoromisonidazole -- 18F-FMISO -- 18F-Fluorocholine -- 18F-FCH -- DCE-US -- CWR22; Medicine, Experimental -- Periodicals; Human experimentation in medicine -- Periodicals; Therapeutics -- Periodicals; 615.50724
• DOI: 10.1186/s12967-015-0708-5
• Languages: English
• ISSNs: 1479-5876

Abstract

Abstract Background This study aims to assess the effect of radiation treatment on the tumour vasculature and its downstream effects on hypoxia and choline metabolism using a multimodal approach in the murine prostate tumour model CWR22. Functional parameters derived from Positron Emission Tomography (PET)/Computer Tomography (CT) with18 F-Fluoromisonidazole (18 F-FMISO) and18 F-Fluorocholine (18 F-FCH) as well as Dynamic Contrast-Enhanced Ultrasound (DCE-US) were employed to determine the relationship between metabolic parameters and microvascular parameters that reflect the tumour microenvironment. Immunohistochemical analysis was employed for validation. Methods PET/CT and DCE-US were acquired pre- and post-treatment, at day 0 and day 3, respectively. At day 1, radiation treatment was delivered as a single fraction of 10 Gy. Two experimental groups were tested for treatment response with18 F-FMISO and18 F-FCH. Results The maximum Standardized Uptake Values (SUVmax) and the mean SUV (SUVmean) for the18 F-FMISO group were decreased after treatment, and the SUVmean of the tumour-to-muscle ratio was correlated to microvessel density (MVD) at day 3. The kurtosis of the amplitude of the contrast uptakeA was significantly decreased for the control tumours in the18 F-FCH group. Furthermore, the eliminating rate constant of the contrast agent from the plasmak el derived from DCE-US was negatively correlated to the SUVmean of tumour-to-muscle ratio, necrosis and MVD. Conclusions The present study suggests that the multimodal approach using18 F-FMISO PET/CT and DCE-US seems reliable in the assessment of both microvasculature and necrosis as validated by histology. Thus, it has valuable diagnostic and prognostic potential for early non-invasive evaluation of radiotherapy.