Authentication of commercial processed Glehniae Radix (Beishashen) by DNA barcodes. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Zhu, Xunzhi; Zhang, Yuxi; Liu, Xia; Hou, Dianyun; Gao, Ting
• Is Part Of: Chinese medicine. Volume 10:Issue 1(2015)
• Page Start: 1
• Page End: 9
• Publication Date: 2015-12
• Subjects: Medicine, Chinese -- Periodicals; Evidence-based medicine -- China -- Periodicals; Medicine, Experimental -- Periodicals; 610.95105
• DOI: 10.1186/s13020-015-0071-8
• Languages: English
• ISSNs: 1749-8546

Abstract

Abstract Background The radix ofGlehnia littoralis Fr. Schmidt ex Miq. (Beishashen ), is often misidentified and adultered in Chinese medicine. Its seven common adulterants includeChuanminshen violaceum Sheh et Shan (Chuanmingshen ), Changium smyrnioides Wolff (Mingdangshen ), Sphallerocarpus gracilis (Bess.) K.-Pol. (Miguoqin ), Adenophora polyantha Nakai (Shishashen ), Silene tatarinowii Regel (Shishengyingzicao ), Adenophora tetraphylla (Thunb.) Fisch (Lunyeshashen ) andAdenophora stricta Miq. (Shashen ). This study aims to evaluate the feasibility of the second internal transcribed spacer (ITS2) DNA barcoding to discriminate betweenGlehniae Radix and its common adulterants. Methods In this study, we collected 46 samples ofG. littoralis and 59 samples of its seven common adulterants. Genomic DNA sequences were extracted from samples, including original plants and commercially processed crude drugs. The ITS2 of the ribosomal DNA sequences were amplified and sequenced bi-directionally. The sequences were assembled by CodonCode Aligner 3.5.7. The descriptive data analysis was conducted and neighbor-joining (NJ) phylogenetic tree was constructed by MEGA 5.0 in accordance with the kimura 2 -parameter (K2P) model. The identification efficiency was evaluated based on the BLAST1 methods. The ITS2 secondary structures were predicted and compared betweenGlehniae Radix and its adulterants by the ITS2 database. Results As the 46 ITS2 sequences ofG. littoralis were identical to each other, the identification efficiency of the ITS2 region was 100 %. A NJ tree based on the ITS2 sequences, and the predicted secondary structures of ITS2, distinguishedGlehniae Radix from its adulterants. Conclusion DNA barcoding based on ITS2 distinguished commercial processedGlehniae Radix from common herbal adulterants.