Identification of Hippophae species (Shaji) through DNA barcodes. Issue 1 (December 2015)

• Record Type: Journal Article
• Authors: Liu, Yue; Sun, Wei; Liu, Chuan; Zhang, Yaqin; Chen, Yilong; Song, Ming; Fan, Gang; Liu, Xia; Xiang, Li; Zhang, Yi
• Is Part Of: Chinese medicine. Volume 10:Issue 1(2015)
• Page Start: 1
• Page End: 11
• Publication Date: 2015-12
• Subjects: Medicine, Chinese -- Periodicals; Evidence-based medicine -- China -- Periodicals; Medicine, Experimental -- Periodicals; 610.95105
• DOI: 10.1186/s13020-015-0062-9
• Languages: English
• ISSNs: 1749-8546

Abstract

Abstract Background The morphological identification of differentHippophae species (Shaji) was difficult. This study aims to discriminate between medicinal and non-medicinalHippophae species by DNA barcodes, the ITS2, psbA -trnH, and a combination of ITS2 andpsbA -trnH (ITS2 + psbA -trnH ). Methods DNA was extracted from the dried fruit samples. Primer pairs ITS2F/3R for ITS2 andpsbA F/trnH R forpsbA -trnH were used for PCR amplification. The purified PCR products were bidirectionally sequenced. Genetic distances were calculated according to the Kimura 2 parameter model and phylogenetic tree was constructed based on neighbor-joining (NJ) method, barcoding gap was also analyzed to assess identification efficiency. Results Amplification and sequencing efficiencies for both ITS2 andpsbA -trnH were 100 %. Sequence data revealed that ITS2 + psbA -trnH was the most suitable candidate barcode at the species and subspecies level. The closely relatedHippophae species were effectively differentiated in the NJ tree. Conclusion The combination of the two loci, ITS2 + psbA -trnH is applicable to the identification of medicinal and non-medicinalHippophae species.