Label-free proteomic analysis of the hydrophobic membrane protein complement in articular chondrocytes: a technique for identification of membrane biomarkers. (17th November 2015)

• Record Type: Journal Article
• Authors: Matta, Csaba; Zhang, Xiaofei; Liddell, Susan; Smith, Julia R.; Mobasheri, Ali
• Is Part Of: Biomarkers. Volume 20:Number 8(2015)
• Page Start: 572
• Page End: 589
• Publication Date: 2015-11-17
• Subjects: Articular cartilage -- chondrocyte -- equine -- mass spectrometry -- membranome -- membrane protein extraction -- proteomics -- Triton X-114; Biochemical markers -- Periodicals; 610.28
• DOI: 10.3109/1354750X.2015.1130191
• Languages: English
• ISSNs: 1354-750X

Abstract

Abstract: Context : There is insufficient knowledge about the chondrocyte membranome and its molecular composition. Objective : To develop a Triton X-114 based separation technique using nanoLC-MS/MS combined with shotgun proteomics to identify chondrocyte membrane proteins. Materials and methods : Articular chondrocytes from equine metacarpophalangeal joints were separated into hydrophobic and hydrophilic fractions; trypsin-digested proteins were analysed by nanoLC-MS/MS. Results : A total of 315 proteins were identified. The phase extraction method yielded a high proportion of membrane proteins (56%) including CD276, S100-A6 and three VDAC isoforms. Discussion : Defining the chondrocyte membranome is likely to reveal new biomarker targets for conventional and biological drug discovery.