Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216T. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Bosma, Elleke; Koehorst, Jasper; van Hijum, Sacha; Renckens, Bernadet; Vriesendorp, Bastienne; van de Weijer, Antonius; Schaap, Peter; de Vos, Willem; van der Oost, John; van Kranenburg, Richard
• Is Part Of: Standards in genomic sciences. Volume 11:Issue 1(2016)
• Page Start: 1
• Page End: 11
• Publication Date: 2016-12
• Subjects: Bacillus smithii -- Genome sequence -- Lactic acid -- Thermophile -- Thermophilic bacillus -- Biotechnology; Genomics -- Periodicals; Genomics -- Periodicals; Genomics; Periodicals; 572.8605
• DOI: 10.1186/s40793-016-0172-8
• Languages: English
• ISSNs: 1944-3277

Abstract

Abstract Bacillus smithii is a facultatively anaerobic, thermophilic bacterium able to use a variety of sugars that can be derived from lignocellulosic feedstocks. Being genetically accessible, it is a potential new host for biotechnological production of green chemicals from renewable resources. We determined the complete genomic sequence of theB. smithii type strain DSM 4216T, which consists of a 3, 368, 778 bp chromosome (GenBank accession number CP012024.1) and a 12, 514 bp plasmid (GenBank accession number CP012025.1), together encoding 3880 genes. Genome annotation via RAST was complemented by a protein domain analysis. Some unique features ofB. smithii central metabolism in comparison to related organisms included the lack of a standard acetate production pathway with no apparent pyruvate formate lyase, phosphotransacetylase, and acetate kinase genes, while acetate was the second fermentation product.