Quantifying heterogeneity and dynamics of clonal fitness in response to perturbation. Issue 7 (July 2015)

• Record Type: Journal Article
• Authors: Frick, Peter L.; Paudel, Bishal B.; Tyson, Darren R.; Quaranta, Vito
• Is Part Of: Journal of cellular physiology. Volume 230:Issue 7(2015:Jul.)
• Page Start: 1403
• Page End: 1412
• Publication Date: 2015-07
• Subjects: Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.24888
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract : The dynamics of heterogeneous clonal lineages within a cell population, in aggregate, shape both normal and pathological biological processes. Studies of clonality typically relate the fitness of clones to their relative abundance, thus requiring long‐term experiments and limiting conclusions about the heterogeneity of clonal fitness in response to perturbation. We present, for the first time, a method that enables a dynamic, global picture of clonal fitness within a mammalian cell population. This novel assay allows facile comparison of the structure of clonal fitness in a cell population across many perturbations. By utilizing high‐throughput imaging, our methodology provides ample statistical power to define clonal fitness dynamically and to visualize the structure of perturbation‐induced clonal fitness within a cell population. We envision that this technique will be a powerful tool to investigate heterogeneity in biological processes involving cell proliferation, including development and drug response. J. Cell. Physiol. 230: 1403–1412, 2015. © 2015 Wiley Periodicals, Inc., A Wiley Company