Boosting of activity enhancement of K+-responsive quadruplex hammerhead ribozyme. Issue 27 (2nd March 2015)

• Record Type: Journal Article
• Authors: Yamaoki, Yudai; Mashima, Tsukasa; Nagata, Takashi; Katahira, Masato
• Is Part Of: Chemical communications. Volume 51:Issue 27(2015)
• Page Start: 5898
• Page End: 5901
• Publication Date: 2015-03-02
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc00961h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Second-generation quadruplex hammerhead ribozymes, whose activity enhances in response to K +, were developed. New strategies suppressed the basal activity and boosted the activity enhancement upon the addition of K + to 21-fold. Abstract : Two second-generation quadruplex hammerhead ribozymes, whose activity enhances in response to K + via quadruplex formation of embedded r(GGA)3 GG, were developed. Different strategies were applied to suppress basal activity when K + is absent. As a result, the activity enhancement upon the addition of K + has reached as high as 21-fold.