Facile purification and click labeling with 2-[18F]fluoroethyl azide using solid phase extraction cartridges. Issue 7 (11th February 2015)

• Record Type: Journal Article
• Authors: Zhou, Dong; Chu, Wenhua; Peng, Xin; McConathy, Jonathan; Mach, Robert H.; Katzenellenbogen, John A.
• Is Part Of: Tetrahedron letters. Volume 56:Issue 7(2015)
• Page Start: 952
• Page End: 954
• Publication Date: 2015-02-11
• Subjects: Fluorine-18 -- Radiolabeling -- Click chemistry -- 2-Fluoroethyl azide -- Positron emission tomography -- Solid phase extraction; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tetlet.2014.10.126
• Languages: English
• ISSNs: 0040-4039

Abstract

Graphical abstract: Abstract: A facile method was developed to purify 2-[ 18 F]fluoroethyl azide ([ 18 F]FEA) using a C18 cartridge and an Oasis® HLB cartridge in series, in which [ 18 F]FEA was exclusively trapped on the HLB cartridge. [ 18 F]FEA can be eluted for reactions in solution; alternatively click labeling can be carried out on the HLB cartridge itself by loading an alkyne substrate and copper (I) catalyst dissolved in DMF onto the cartridge. This solid phase extraction methodology for purification and click labeling with [ 18 F]FEA, either in solution or on the cartridge, is safe, simple, reproducible in high yield, and compatible with automated synthesis of 18 F-labeled PET tracers.