Characterization and evaluation of antibacterial and antiproliferative activities of crude protein extracts isolated from the seed of Ricinus communis in Bangladesh. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Al-Mamun, M.; Akter, Zerin; Uddin, Md; Ferdaus, K.; Hoque, K.; Ferdousi, Z.; Reza, M.
• Is Part Of: BMC complementary and alternative medicine. Volume 16:Issue 1(2016)
• Page Start: 1
• Page End: 10
• Publication Date: 2016-12
• Subjects: Apoptosis -- Castor bean -- Disc diffusion -- EAC cells -- Lectin -- MIC -- Mice -- Protein; Alternative medicine -- Periodicals; Complementary Therapies -- Periodicals; 615.505
• DOI: 10.1186/s12906-016-1185-y
• Languages: English
• ISSNs: 1472-6882

Abstract

Abstract Background Ricinus communis (Euphorbiaceae ) has previously been reported to possess analgesic, antihistamine, antioxidant and anti-inflammatory activities. This study was designed for isolation, characterization and evaluation of antibacterial and anti-proliferative activities ofR. communis seed protein. Methods The concentration and molecular weight ofR. communis seed protein were estimated by SDS-PAGE and spectrophotometric analysis, respectively. Lectin activity was evaluated by hemagglutination assay on mice blood. In vitro susceptibility of four human pathogenic bacteria includingEscherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes andStaphylococcus aureus was detected using disk diffusion assay, and minimum inhibitory concentration (MIC) value was determined using micro-dilution method. A total of twenty four Swiss albino mice containing Ehrlich's ascites carcinoma (EAC) cells were treated with the crude protein ofR. communis at 50 and 100 μg/ml/d/mouse for 6 days. Growth inhibitory activity ofR. communis seed protein on EAC cells was determined by haemocytometer counting using trypan blue dye and DAPI (4΄, 6-diamidino-2-phenylindole) staining was used to assess apoptotic cells. Results The protein concentration of sixR. communis (castor) varieties ranged between 21–35 mg/ml and molecular weight between 14–200 kDa. Castor protein agglutinated mice blood at 3.125 μg/wall. The seed protein shows considerable antimicrobial activity againstE. coli, P. aeruginosa andS. aureus, exhibiting MIC values of 250, 125 and 62.5 μg/ml, respectively. Administration of seed protein led to 54 % growth inhibition of EAC cells at 100 μg/ml. DAPI staining indicates marked features of apoptosis including condensation of cytoplasm, nuclear fragmentation and aggregation of apoptotic bodies etc. Conclusion Our study suggests that the lectin richR. communis seed protein has strong antibacterial and anticancer activities.