Progerin, the protein responsible for the Hutchinson-Gilford progeria syndrome, increases the unrepaired DNA damages following exposure to ionizing radiation. (December 2015)

• Record Type: Journal Article
• Authors: Noda, Asao; Mishima, Shuji; Hirai, Yuko; Hamasaki, Kanya; Landes, Reid; Mitani, Hiroshi; Haga, Kei; Kiyono, Tohru; Nakamura, Nori; Kodama, Yoshiaki
• Is Part Of: Genes and environment. Volume 37:Number 1(2015)
• Page Start: 1
• Page End: 12
• Publication Date: 2015-12
• Subjects: HGPS -- Unrepairable DSB -- Radiation -- Cell aging -- FTI; Mutagenesis -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Mutagens -- Periodicals; Mutation (Biology) -- Periodicals; Genetic toxicology -- Periodicals; Genetic toxicology; Mutagenesis; Mutagens; Mutation (Biology); Pollution -- Environmental aspects; Periodicals; 572.838
• DOI: 10.1186/s41021-015-0018-4
• Languages: English
• ISSNs: 1880-7062

Abstract

Abstract Introduction Progerin, the protein responsible for the Hutchinson-Gilford Progeria Syndrome (HGPS), is a partially deleted form of nuclear lamin A, and its expression has been suggested as a cause for dysfunctional nuclear membrane and premature senescence. To examine the role of nuclear envelop architecture in regulating cellular aging and DNA repair, we used ionizing radiation to increase the number of DNA double strand breaks (DSBs) in normal and HGPS cells, and analyzed possible relationship between unrepaired DSBs and cellular aging. Results We found that HGPS cells are normal in repairing a major fraction of radiation-induced double strand breaks (M-DSBs)but abnormal to show increased amount of residual unrepaired DSBs (R-DSBs). Such unrepaired DSBs were 2.6 times (CI 95 %: 2.2–3.2) higher than that in normal cells one week after the irradiation, and 1.6 times (CI 95 %: 1.3–1.9) higher even one month after the irradiation. These damages tend to increase as the nuclear envelope become abnormal, a characteristic of both HGPS and normal human cells which undergo replicative senescence. The artificial, enforced over-expression of progerin further impaired the repair of M-DSBs, implying lamin A-associated nuclear membrane has an important role for DNA DSB repair. Introduction of telomerase gene function in HGPS cells reversed such aging phenotypes along with upregulation of lamin B1 and downregulation of progerin, which is a hallmark of young cells. Conclusion We suggest that lamin A- or progerin-associated nuclear envelope is involved in cellular aging associated with DNA damage repair.