Cell viability, reactive oxygen species, apoptosis, and necrosis in myoblast cultures exposed to low-level infrared laser. Issue 5 (July 2016)

• Record Type: Journal Article
• Authors: Alexsandra da Silva Neto Trajano, Larissa; da Silva, Camila; de Carvalho, Simone; Cortez, Erika; Mencalha, André; de Souza da Fonseca, Adenilson; Stumbo, Ana
• Is Part Of: Lasers in medical science. Volume 31:Issue 5(2016)
• Page Start: 841
• Page End: 848
• Publication Date: 2016-07
• Subjects: Apoptosis -- Cell viability -- Reactive oxygen species -- Low-level laser therapy -- Myoblast -- Necrosis; Lasers in medicine -- Periodicals; 610.28
• DOI: 10.1007/s10103-016-1909-8
• Languages: English
• ISSNs: 0268-8921

Abstract

Abstract Low-level infrared laser is considered safe and effective for treatment of muscle injuries. However, the mechanism involved on beneficial effects of laser therapy are not understood. The aim was to evaluate cell viability, reactive oxygen species, apoptosis, and necrosis in myoblast cultures exposed to low-level infrared laser at therapeutic fluences. C2C12 myoblast cultures at different (2 and 10 %) fetal bovine serum (FBS) concentrations were exposed to low-level infrared laser (808 nm, 100 mW) at different fluences (10, 35, and 70 J/cm2 ) and evaluated after 24, 48, and 72 h. Cell viability was evaluated by WST-1 assay; reactive oxygen species (ROS), apoptosis, and necrosis were evaluated by flow cytometry. Cell viability was decreased atthe lowest FBS concentration. Laser exposure increased the cell viability in myoblast cultures at 2 % FBS after 48 and 72 h, but no significant increase in ROS was observed. Apoptosis was decreased at the higher fluence and necrosis was increased at lower fluence in myoblast cultures after 24 h of laser exposure at 2 % FBS. No laser-induced alterations were obtained at 10 % FBS. Results show that level of reactive oxygen species is not altered, at least to those evaluated in this study, but low-level infrared laser exposure affects cell viability, apoptosis, and necrosis in myoblast cultures depending on laser fluence and physiologic conditions of cells.