Extended phylogenetic analysis of a new Israeli isolate of Brevicoryne brassicae virus (BrBV-IL) suggests taxonomic revision of the genus Iflavirus. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Luria, Neta; Reingold, Victoria; Lachman, Oded; Sela, Noa; Dombrovsky, Aviv
• Is Part Of: Virology journal. Volume 13:Issue 1(2016)
• Page Start: 1
• Page End: 5
• Publication Date: 2016-12
• Subjects: Iflavirus -- Aphid-infecting virus; Virology -- Periodicals; 579.2
• DOI: 10.1186/s12985-016-0500-z
• Languages: English
• ISSNs: 1743-422X

Abstract

Abstract Background Brevicoryne brassicae virus (BrBV) is a positive-strand genomic RNA virus which is unassigned tentative member of the genusIflavirus . BrBv was first identified and characterized in the late 90's in the cabbage aphid in the United Kingdom (UK) (J Gen Virol 88:2590–2595, 2007) and was fully sequenced, using random amplification of encapsidated RNA. No other reports have been published demonstrating detection of this virus outside the UK. Findings A new isolate of the cabbage aphid virusBrevicoryne brassicae virus was identified fromBrevicoryne brassicae aphids growing on wild mustard plants (Sinapis arvensis ) in northern Israel. The virus genome was partially assembled from purified siRNA using the Illumina MiSeq Sequencing System with limited success. Combining classical viral RNA purification and RT-PCR amplification followed by traditional Sanger sequencing enabled obtaining the complete genomic sequence. The Israeli strain of BrBV shared 95 % nucleotide sequence identity with the BrBV found in the United Kingdom. Conclusions The detection of BrBV in Israel indicates a broader geographical distribution of the virus".