Ex vivo expansion of circulating lung tumor cells based on one-step microfluidics-based immunomagnetic isolation. Issue 12 (18th February 2016)

• Record Type: Journal Article
• Authors: Wang, Zhihua; Wu, Wenjun; Wang, Zhuo; Tang, Ying; Deng, Yuliang; Xu, Ling; Tian, Jianhui; Shi, Qihui
• Is Part Of: Analyst. Volume 141:Issue 12(2016)
• Page Start: 3621
• Page End: 3625
• Publication Date: 2016-02-18
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c5an02554k
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : This paper reports a method to preserve the high viability of CTCs and enrich isolated CTCs in a small volume of culture medium for successful ex vivo expansion. Abstract : We describe a one-step microfludics-based immunomagnetic isolation method to isolate CTCs directly from the whole blood of lung adenocarcinoma patients. This method avoids harsh sample preparation and enrichment steps, and therefore preserves the viability of CTCs during the in vitro isolation. Importantly, isolated, magnetic bead-bearing CTCs are concentrated in a small volume of culture medium with a high CTC density. High cell viability and culturing density promote the ex vivo expansion of limited numbers of CTCs. Expanded CTCs are characterized at the genetic, protein and metabolic levels.