Antioxidant activity and peroxidase inhibition of Amazonian plants extracts traditionally used as anti-inflammatory. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: de Vargas, Fabiano; Almeida, Patricia; de Boleti, Ana; Pereira, Maria; de Souza, Tatiane; de Vasconcellos, Marne; Nunez, Cecilia; Pohlit, Adrian; Lima, Emerson
• Is Part Of: BMC complementary and alternative medicine. Volume 16:Issue 1(2016)
• Page Start: 1
• Page End: 8
• Publication Date: 2016-12
• Subjects: Amazon medicinal plants -- Antioxidants -- Citotoxicity -- Reactive oxygen species -- Free radical scavenging; Alternative medicine -- Periodicals; Complementary Therapies -- Periodicals; 615.505
• DOI: 10.1186/s12906-016-1061-9
• Languages: English
• ISSNs: 1472-6882

Abstract

Abstract Background The Amazon is the largest rainforest in the world and is home to a rich biodiversity of medicinal plants. Several of these plants are used by the local population for the treatment of diseases, many of those with probable anti-inflammatory effect. The aim of the present investigation was to evaluate the in vitro antioxidant and anti-peroxidases potential of the ethanol extracts of five plants from the Brazilian Amazon (Byrsonima japurensis, Calycophyllum spruceanum, Maytenus guyanensis, Passiflora nitida andPtychopetalum olacoides ). Methods DPPH, ABTS, superoxide anion radical, singlet oxygen and the β-carotene bleaching methods were employed for characterization of free radical scavenging activity. Also, total polyphenols were determined. Antioxidant activities were evaluated using murine fibroblast NIH3T3 cell. Inhibition of HRP and MPO were evaluated using amplex red® as susbtract. Results The stem bark extracts ofC. spruceanum andM. guyanensis provided the highest free radical scavenging activities.C. spruceanum exhibited IC50 = 7.5 ± 0.9, 5.0 ± 0.1, 18.2 ± 3.0 and 92.4 ± 24.8 μg/mL for DPPH, ABTS+, O2 - and1 O2 assays, respectively.P. olacoides andC. spruceanum extracts also inhibited free radicals formation in the cell-based assay. At a concentration of 100 μg/mL, the extracts ofC. spruceanum, B. japurensis inhibited horseradish peroxidase by 62 and 50 %, respectively.C. spruceanum, M. guyanensis, B. japurensis also inhibited myeloperoxidase in 72, 67 and 56 %, respectively. Conclusions This work supports the folk use these species that inhibited peroxidases and exhibited significant free radical scavenging and antioxidant activities what can be related to treatment of inflammation.