In vitro and in vivo reduction of post-prandial blood glucose levels by ethyl alcohol and water Zingiber mioga extracts through the inhibition of carbohydrate hydrolyzing enzymes. Issue 1 (December 2016)

• Record Type: Journal Article
• Authors: Jo, Sung-Hoon; Cho, Cha-Young; Lee, Jung-Yoon; Ha, Kyoung-Soo; Kwon, Young-In; Apostolidis, Emmanouil
• Is Part Of: BMC complementary and alternative medicine. Volume 16:Issue 1(2016)
• Page Start: 1
• Page End: 7
• Publication Date: 2016-12
• Subjects: α-glucosidase -- Sucrase -- Anti-hyperglycemia -- Blood glucose -- Oxygen radical absorbance capacity -- Zingiber mioga; Alternative medicine -- Periodicals; Complementary Therapies -- Periodicals; 615.505
• DOI: 10.1186/s12906-016-1090-4
• Languages: English
• ISSNs: 1472-6882

Abstract

Abstract Background Type 2 diabetes is a serious problem for developed and developing countries. Prevention of prediabetes progression to type 2 diabetes with the use of natural products appears to be a cost-effective solution.Zingiber mioga has been used as a traditional food in Asia. Recent research has reported the potential health benefits ofZingiber mioga, but the blood glucose reducing effect has not been yet evaluated. Methods In this studyZingiber mioga extracts (water and ethanol) were investigated for their anti-hyperglycemic and antioxidant potential using both in vitro and animal models. The in vitro study evaluated the total phenolic content, the oxygen radical absorbance capacity (ORAC) and the inhibitory effect against carbohydrate hydrolyzing enzymes (porcine pancreatic α-amylase and rat intestinal sucrase and maltase) of bothZingiber mioga extracts. Also, the extracts were evaluated for their in vivo post-prandial blood glucose reducing effect using SD rat anddb/db mice models. Results Our findings suggest that the ethanol extract ofZingiber mioga (ZME) exhibited the higher sucrase and maltase inhibitory activity (IC50, 3.50 and 3.13 mg/mL) and moderate α-amylase inhibitory activity (IC50, >10 mg/mL). Additionally, ZME exhibited potent peroxyl radical scavenging linked antioxidant activity (0.53/TE 1 μM). The in vivo study using SD rat anddb/db mice models also showed that ZME reduces postprandial increases of blood glucose level after an oral administration of sucrose by possibly acting as an intestinal α-glucosidase inhibitor (ZME 0.1 g/kg 55.61 ± 13.24 mg/dL) Conclusion The results indicate thatZingiber mioga extracts exhibited significant in vitro α-glucosidase inhibition and antioxidant activity. Additionally, the tested extracts demonstrated in vivo anti-hyperglycemic effects using SD rat anddb/db mice models. Our findings provide a strong rationale for the further evaluation ofZingiber mioga for the potential to contribute as a useful dietary strategy to manage postprandial hyperglycemia.